Saito Hiroaki, Yamada Yoshinori, Takaya Seigo, Osaki Tomohiro, Ikeguchi Masahide
Department of Surgery, Division of Surgical Oncology, Tottori University School of Medicine, 36-1 Nishi-cho, Yonago, 683-8504, Japan.
Surg Today. 2015 Nov;45(11):1429-35. doi: 10.1007/s00595-015-1165-8. Epub 2015 Apr 14.
There is accumulating evidence that inflammation is linked to cancer development and progression. Interleukin-17 (IL-17), an inflammatory cytokine, is produced by CD 8+ T cells (Tc17 cells); however, the specific role of Tc17 cells in tumor immunity against gastric cancer remains unclear.
The prevalence of Tc17 cells in both peripheral blood mononuclear cells and gastric tissue was evaluated by multicolor flow cytometry and the concentration of IL-17 in sera was quantitated by an enzyme-linked immunosorbent assay.
Circulating Tc17 cells were significantly more numerous in gastric cancer patients than in controls, and significantly more numerous before surgery than after surgery. IL-17 concentrations in gastric cancer patients and healthy controls were 0.51 ± 0.54 and 0.084 ± 0.084 pg/mL, respectively, with this difference being significant. The percentage of Tc17 cells was significantly related to serum IL-17 concentration. Tc17 cells were significantly more numerous than peripheral blood mononuclear cells in gastric cancer tissue. Furthermore, Tc17 cells were more numerous in cancerous gastric tissue than in normal gastric tissue.
Tc17 cells may be the main source of IL-17 in gastric cancer patients and thus involved in the progression of gastric cancer.
越来越多的证据表明炎症与癌症的发生和发展有关。白细胞介素-17（IL-17）作为一种炎性细胞因子，由CD8 + T细胞（Tc17细胞）产生；然而，Tc17细胞在胃癌肿瘤免疫中的具体作用仍不清楚。
采用多色流式细胞术评估外周血单个核细胞和胃组织中Tc17细胞的比例，并用酶联免疫吸附测定法定量血清中IL-17的浓度。
胃癌患者循环中的Tc17细胞数量显著多于对照组，且术前明显多于术后。胃癌患者和健康对照者血清IL-17浓度分别为0.51±0.54和0.084±0.084 pg/mL，差异有统计学意义。Tc17细胞比例与血清IL-17浓度显著相关。胃癌组织中Tc17细胞数量明显多于外周血单个核细胞。此外，癌性胃组织中的Tc17细胞比正常胃组织中的更多。
Tc17细胞可能是胃癌患者IL-17的主要来源，从而参与胃癌的进展。
