改良的欧洲抗菌药物敏感性试验委员会（EUCAST）菌丝体片段接种法用于皮肤癣菌体外药敏试验及新型抗真菌药物活性的评估
Evaluation of a modified EUCAST fragmented-mycelium inoculum method for in vitro susceptibility testing of dermatophytes and the activity of novel antifungal agents.
作者信息
Risslegger B, Lass-Flörl C, Blum G, Lackner M
机构信息
Department of Hygiene, Microbiology and Social Medicine, Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria.
Department of Hygiene, Microbiology and Social Medicine, Division of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria
出版信息
Antimicrob Agents Chemother. 2015;59(6):3675-82. doi: 10.1128/AAC.04381-14. Epub 2015 Apr 13.
Abstract
For antifungal susceptibility testing of nonsporulating or poorly sporulating dermatophytes, a fragmented-mycelium inoculum preparation method was established and compared to broth microdilution testing according to CLSI and EUCAST guidelines. Moreover, the in vitro activity of new antifungal agents against dermatophytes was evaluated. Agreement between the mycelial inoculum method and the CLSI broth microdilution method was high (93% to 100%). Echinocandins (minimal effective concentration [MEC], ≤0.5 mg/liter) and posaconazole (MIC, ≤3.00 mg/liter) showed good activity against all tested dermatophytes.
摘要
对于非产孢或产孢不良的皮肤癣菌进行抗真菌药敏试验，建立了一种碎菌丝接种体制备方法，并根据CLSI和EUCAST指南与肉汤微量稀释试验进行比较。此外，评估了新型抗真菌药物对皮肤癣菌的体外活性。菌丝接种法与CLSI肉汤微量稀释法之间的一致性很高（93%至100%）。棘白菌素（最低有效浓度[MEC]，≤0.5毫克/升）和泊沙康唑（MIC，≤3.00毫克/升）对所有测试的皮肤癣菌均显示出良好活性。
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