用于两性霉素B药敏试验的曲霉菌丝接种物标准化
Standardization of a hyphal inoculum of aspergilli for amphotericin B susceptibility testing.
作者信息
Bezjak V
出版信息
J Clin Microbiol. 1985 Apr;21(4):509-12. doi: 10.1128/jcm.21.4.509-512.1985.
Abstract
Standardized, evenly dispersed hyphal suspensions served as the inoculum in a microtiter technique for amphotericin B antifungal susceptibility testing. Preliminary testing with six strains of Aspergillus fumigatus and A. flavus produced consistent and reproducible results at 30 degrees C over 24 h. The observed amphotericin B MICs required for hyphae (0.3 to 0.6 microgram/ml) were comparable to MICs required for conidia (0.16 to 0.6 microgram/ml). The results were evaluated and compared with previously published information.
摘要
在两性霉素B抗真菌药敏试验的微量滴定技术中，标准化、均匀分散的菌丝悬浮液用作接种物。对6株烟曲霉和黄曲霉进行的初步试验在30℃下24小时内产生了一致且可重复的结果。观察到的菌丝所需两性霉素B的最低抑菌浓度（MIC）（0.3至0.6微克/毫升）与分生孢子所需的MIC（0.16至0.6微克/毫升）相当。对结果进行了评估，并与先前发表的信息进行了比较。
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