Lăzărescu H, Simionca I, Hoteteu M, Mirescu L
National Institute of Rehabilitation, Physical Medicine and Balneoclimatology, Bucharest, Romania.
J Med Life. 2014;7 Spec No. 2(Spec Iss 2):76-9.
Speleotherapy - a special form of climatotherapy - uses certain conditions specific to caves and salt-mines to treat several medical conditions, especially respiratory and skin-related. This reduces all types of irritations and therefore disease symptoms are mitigated or fully suppressed while the patient is accommodated into the salt-mine.
Influence of microclimate in salt-mines of Turda, Dej and Cacica on morphology and electrophoretic expression of in vitro lung and skin fibroblasts acquired from the lung and hypodermic tissues of Wistar rats, in normal conditions and after ovalbumin-induced asthma, respectively after experimental injuries and burns.
skin fibroblast cultures acquired from lung and hypodermic tissue sampled from Wistar rats. Cultures acquired are developed in fibroblast monolayer attached to the culture dish. Wistar rats with weight between 75 -100 g were divided in three groups: one control group, one group with experimental asthma, one group with injuries and burns. 10 animals from each group were sent to salt-mines in Turda, Dej and Cacica for 14 days and kept in a saline environment, similar to speleotherapy.
Speleotherapy applied to Wistar rats determined significant differences in cellular morphology and in electrophoretic expression of lung and skin fibroblasts from primary cultures.
Results of this survey indicates that speleotherapy induces changes in morphology and protein expression of in vitro lung and skin fibroblasts, and these changes support the therapeutic effects of speleotherapy.
洞穴疗法——气候疗法的一种特殊形式——利用洞穴和盐矿特有的某些条件来治疗多种疾病，尤其是与呼吸和皮肤相关的疾病。这能减轻所有类型的刺激，因此在患者置身于盐矿时，疾病症状会减轻或完全得到抑制。
分别在正常条件下以及卵清蛋白诱导哮喘后、实验性损伤和烧伤后，研究图尔达、德日和卡奇察盐矿的微气候对从Wistar大鼠肺和皮下组织获取的体外肺和皮肤成纤维细胞的形态和电泳表达的影响。
从Wistar大鼠的肺和皮下组织获取皮肤成纤维细胞培养物。所获取的培养物在附着于培养皿的成纤维细胞单层中生长。体重在75 - 100克之间的Wistar大鼠分为三组：一组为对照组，一组为实验性哮喘组，一组为损伤和烧伤组。每组10只动物被送往图尔达、德日和卡奇察的盐矿14天，并置于类似洞穴疗法的盐环境中。
对Wistar大鼠应用洞穴疗法后，原代培养的肺和皮肤成纤维细胞在细胞形态和电泳表达方面出现了显著差异。
本次调查结果表明，洞穴疗法可诱导体外肺和皮肤成纤维细胞的形态和蛋白质表达发生变化，且这些变化支持洞穴疗法的治疗效果。
