Runtuwene Joshua, Amitani Haruka, Amitani Marie, Asakawa Akihiro, Cheng Kai-Chun, Inui Akio
Department of Psychosomatic Internal Medicine, Kagoshima University Graduate School of Medical and Dental Sciences , Kagoshima , Japan ; Faculty of Medicine, Sam Ratulangi University , Manado , Indonesia.
Department of Psychosomatic Internal Medicine, Kagoshima University Graduate School of Medical and Dental Sciences , Kagoshima , Japan.
PeerJ. 2015 Apr 7;3:e859. doi: 10.7717/peerj.859. eCollection 2015.
Oxidative stress is involved in cancer development. Hydrogen (H2) is a potent antioxidant and exhibits anti-inflammatory and potentially anticancer-like activities. This study aimed to investigate the role of H2 incombination with 5-fluorouracil (5-FU) in cancer treatment both in vitro and in vivo using the colon 26 cell line. The survival rate was determined using the Kaplan-Meier survival test, and cell viability was assessed using cell viability imaging kit and the MTT assay, and activation of the cell apoptosis pathway (Phosphorylated adenosine monophosphate activated protein kinase (p-AMPK), Apoptosis-inducing factor (AIF) and Caspase 3) were characterized by western blots. Hydrogen water administration improved the survival of mice with colon 26-induced cancer. Furthermore, hydrogen water enhanced cell apoptosis in cancer cells, resulting in a marked increase in the expression of p-AMPK, AIF and Caspase 3 in colon 26 cells. Hydrogen water also increased the inhibitory effect of 5-FU on colon 26 cells with spect to cell survival rate and anticancer functions. Additionally, high-content hydrogen water exhibited stronger antioxidative and anticancer activity than did the natural hydrogen water. In conclusion, high-content hydrogen water can inhibit colon cancer, particularly in combination with 5-fluorouracil.
氧化应激参与癌症发展。氢气（H₂）是一种强效抗氧化剂，具有抗炎和潜在的抗癌样活性。本研究旨在利用结肠26细胞系在体外和体内研究氢气与5-氟尿嘧啶（5-FU）联合在癌症治疗中的作用。使用Kaplan-Meier生存试验确定生存率，使用细胞活力成像试剂盒和MTT试验评估细胞活力，并通过蛋白质印迹法表征细胞凋亡途径（磷酸化腺苷单磷酸活化蛋白激酶（p-AMPK）、凋亡诱导因子（AIF）和半胱天冬酶3）的激活情况。给予氢水可提高结肠26诱导的癌症小鼠的生存率。此外，氢水增强癌细胞中的细胞凋亡，导致结肠26细胞中p-AMPK、AIF和半胱天冬酶3的表达显著增加。氢水还增强了5-FU对结肠26细胞的细胞生存率和抗癌功能的抑制作用。此外，高浓度氢水比天然氢水表现出更强的抗氧化和抗癌活性。总之，高浓度氢水可抑制结肠癌，尤其是与5-氟尿嘧啶联合使用时。
