Najafipour Reza, Moghbelinejad Sahar, Samimi Hashjin Amir, Rajaei Farzad, Rashvand Zahra
Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran ; Department of Medical Genetics, Qazvin University of Medical Sciences, Qazvin, Iran.
Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran.
Cell J. 2015 Spring;17(1):121-8. doi: 10.22074/cellj.2015.518. Epub 2015 Apr 8.
Animal model studies have shown that MSY2 and JHDM2A genes have an important role in spermatogenesis process and fertility of male mice. But the potential role of these genes in human spermatogenesis and fertility is not known yet. Therefore, we evaluated expression ratios of these genes in testis tissues of men with normal and impaired spermatogenesis.
In this experimental study, after RNA extraction and cDNA syn- thesis from 50 non-obstructive azoospermic and 12 normal testis tissues, the expression ratios of genes were evaluated by real time polymerase chain reaction (PCR) technique. Hematoxcylin and eosin (H&E) staining was used for histological classification of testis tissues. For statistical analysis, one way analysis of variance (ANOVA) test was carried out.
Our results showed a significant reduction in mRNA level of YBX2 in samples with impaired spermatogenesis (p<0.001) compared to samples with qualitatively normal spermatogenesis and normal spermatogenesis; however, in JHDM2A gene, despite sensible reduction in gene expression level in men with impaired spermatogenesis, no significant differences were shown (p>0.05). Furthermore in YBX2, a significant negative correlation was demonstrated between the efficiency score of spermatogenesis and the threshold cycle (CT) (r=-0.7, p<0.0001), whereas in JHDM2A, this negative correlation was not significant (r=-0.4, p=0.06).
Generally, these data indicated that YBX2 and JHDM2A genes may play an important role in male infertility, and suggested that these molecules can act as useful biomarkers for predicting male infertility.
动物模型研究表明，MSY2和JHDM2A基因在雄性小鼠的精子发生过程和生育能力中起重要作用。但这些基因在人类精子发生和生育能力中的潜在作用尚不清楚。因此，我们评估了这些基因在精子发生正常和受损男性睾丸组织中的表达比率。
在本实验研究中，从50例非梗阻性无精子症患者和12例正常睾丸组织中提取RNA并合成cDNA后，采用实时聚合酶链反应（PCR）技术评估基因的表达比率。苏木精和伊红（H&E）染色用于睾丸组织的组织学分类。进行单因素方差分析（ANOVA）检验以进行统计分析。
我们的结果显示，与精子发生质量正常和精子发生正常的样本相比，精子发生受损样本中YBX2的mRNA水平显著降低（p<0.001）；然而，在JHDM2A基因中，尽管精子发生受损男性的基因表达水平明显降低，但未显示出显著差异（p>0.05）。此外，在YBX2中，精子发生效率评分与阈值循环（CT）之间存在显著负相关（r=-0.7，p<0.0001），而在JHDM2A中，这种负相关不显著（r=-0.4，p=0.06）。
总体而言，这些数据表明YBX2和JHDM2A基因可能在男性不育中起重要作用，并表明这些分子可作为预测男性不育的有用生物标志物。
