基于1.6兆赫兹频域锁模扫频光源实现的4D光学相干断层扫描微血管造影术。
4D optical coherence tomography-based micro-angiography achieved by 1.6-MHz FDML swept source.
作者信息
Zhi Zhongwei, Qin Wan, Wang Jingang, Wei Wei, Wang Ruikang K
出版信息
Opt Lett. 2015 Apr 15;40(8):1779-82. doi: 10.1364/OL.40.001779.
Abstract
We demonstrate the use of an ultra-high-speed swept-source optical coherence tomography (OCT) to achieve optical micro-angiography (OMAG) of microcirculatory tissue beds in vivo. The system is based on a 1310-nm Fourier domain mode-locking (FDML) laser with 1.6-MHz A-line rate, providing a frame rate of 3.415 KHz, an axial resolution of ∼10 μm and signal to noise ratio of 102 dB. Motion from blood flow causes change in OCT signals between consecutive B-frames acquired at the same location. Intensity-based inter-frame subtraction algorithm is applied to extract blood flow from tissue background without any motion correction. We demonstrate the capability of this 1.6-MHz OCT system for 4D OMAG of in vivo tissue at a volume rate of 4.7 volumes/s (volume size: 512×200×720 voxels).
摘要
我们展示了使用超高速扫频源光学相干断层扫描（OCT）在体内实现微循环组织床的光学微血管造影（OMAG）。该系统基于一台1310纳米的傅里叶域锁模（FDML）激光器，A线速率为1.6兆赫兹，帧率为3.415千赫兹，轴向分辨率约为10微米，信噪比为102分贝。血流运动导致在同一位置采集的连续B帧之间的OCT信号发生变化。基于强度的帧间减法算法被应用于从组织背景中提取血流，无需任何运动校正。我们展示了这个1.6兆赫兹OCT系统以4.7体积/秒的体积速率对体内组织进行4D OMAG的能力（体积大小：512×200×720体素）。
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Invest Ophthalmol Vis Sci. 2014 Feb 20;55(2):1024-30. doi: 10.1167/iovs.13-12864.
3
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J Biomed Opt. 2014 Feb;19(2):021105. doi: 10.1117/1.JBO.19.2.021105.
4
Graphics processing unit accelerated optical coherence tomography processing at megahertz axial scan rate and high resolution video rate volumetric rendering.
J Biomed Opt. 2013 Feb;18(2):26002. doi: 10.1117/1.JBO.18.2.026002.
5
Using ultrahigh sensitive optical microangiography to achieve comprehensive depth resolved microvasculature mapping for human retina.
J Biomed Opt. 2011 Oct;16(10):106013. doi: 10.1117/1.3642638.
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Biomed Opt Express. 2011 Apr 1;2(5):1059-68. doi: 10.1364/BOE.2.001059.
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