[三氧化矿物凝聚体对乳牙和恒牙人牙髓细胞增殖与分化的影响]

[Effects of mineral trioxide aggregate on the proliferation and differentiation of human pulp cells from primary and permanent teeth].

作者信息

Minyong Wang, He Liu, Shenglin Li, Man Qin

出版信息

Hua Xi Kou Qiang Yi Xue Za Zhi. 2015 Feb;33(1):75-9. doi: 10.7518/hxkq.2015.01.017.

DOI:10.7518/hxkq.2015.01.017

PMID:25872304

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7030245/

Abstract

OBJECTIVE

This study aims to investigate the effects of mineral trioxide aggregate (MTA) and calcium hydroxide on proliferation and differentiation of human pulp cells from primary and permanent teeth.

METHODS

Cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. The mRNA expression levels of dentinogenesis-related factors, including alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP), and odontoclastogenesis-related factors, such as osteo- protegerin (OPG) and receptor activator of NF-κB ligand (RANKL), were determined by real time polymerase chain reac- tion (PCR).

RESULTS

Primary and permanent pulp cells treated with calcium hydroxide exhibited significantly lower proli- feration rates than the control cells (P<0.01). By contrast, the MTA-treated group showed significantly higher proliferation rates than the control group (P<0.01). Real time PCR results showed that calcium hydroxide-treated primary pulp cells exhi- bited significantly decreased ALP, DSPP, and OPG expression compared with the control group (P<0.01). Conversely, the MTA-treated group displayed significantly increased ALP, DSPP, and OPG expression (P<0.01). Calcium hydroxide-treated primary pulp cells also exhibited significantly upregulated RANKL expression (P < 0.01); by contrast, MTA-treated cells did not show any change in RANKL expression (P>0.05). Likewise, MTA-treated permanent pulp cells showed significantly upregulated ALP and DSPP expression (P < 0.01). However, the calcium hydroxide-treated group remained almost the same as the control group (P > 0.05). Neither MTA nor calcium hydroxide affected OPG and RANKL expression in per- manent pulp cells (P > 0.05).

CONCLUSION

MTA is more suitable as a pulp-capping agent, particularly in primary teeth, than calcium hydroxide.

摘要

目的

本研究旨在探讨三氧化矿物凝聚体（MTA）和氢氧化钙对乳牙和恒牙人牙髓细胞增殖和分化的影响。

方法

采用甲基噻唑基四氮唑（MTT）法检测细胞增殖。通过实时聚合酶链反应（PCR）测定牙本质生成相关因子，包括碱性磷酸酶（ALP）和牙本质涎磷蛋白（DSPP），以及破牙细胞生成相关因子，如骨保护素（OPG）和核因子κB受体活化因子配体（RANKL）的mRNA表达水平。

结果

用氢氧化钙处理的乳牙和恒牙牙髓细胞的增殖率显著低于对照细胞（P<0.01）。相比之下，MTA处理组的增殖率显著高于对照组（P<0.01）。实时PCR结果显示，与对照组相比，用氢氧化钙处理的乳牙牙髓细胞中ALP、DSPP和OPG的表达显著降低（P<0.01）。相反，MTA处理组中ALP、DSPP和OPG的表达显著增加（P<0.01）。用氢氧化钙处理的乳牙牙髓细胞中RANKL的表达也显著上调（P<0.01）；相比之下，MTA处理的细胞中RANKL的表达没有变化（P>0.05）。同样，MTA处理的恒牙牙髓细胞中ALP和DSPP的表达显著上调（P<0.01）。然而，氢氧化钙处理组与对照组几乎相同（P>0.05）。MTA和氢氧化钙均未影响恒牙牙髓细胞中OPG和RANKL的表达（P>0.05）。