Lv Qing-Bo, Gao Xiang, Lin Ding-Sheng, Chen Yun, Cao Bin, Zhou Kai-Liang
Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China.
Biomed Rep. 2016 Sep;5(3):383-389. doi: 10.3892/br.2016.733. Epub 2016 Aug 2.
Partial necrosis of skin flaps continues to restrict the survival of local skin flaps following plastic and reconstructive surgeries. The aim of the present study was to investigate the effects of diammonium glycyrrhizinate (DG), a salt of glycyrrhetinic acid that has been widely used in the therapy of chronic hepatitis and human immunodeficiency virus infection, on random skin flap survival in rats. McFarlane flaps were established in 60 male Sprague-Dawley rats randomly divided into three groups. Group I served as the control group and was injected with saline (10 mg/kg) once per day. Group II and group III were the experimental groups, and were injected with 10 mg/kg DG once and twice per day, respectively. On day 7, the survival area of the flap was measured. Tissue samples were stained with hematoxylin and eosin and immunohistochemically evaluated. Tissue edema, neutrophil density, superoxide dismutase (SOD) activity and malondialdehyde (MDA) levels were evaluated. The mean survival areas of the flaps of group II were significantly larger when compared with those of group I (P<0.05), and the rats of group III exhibited significantly higher survival areas than group II (P<0.05). Histologic and immunohistochemical evaluation showed that microvessel development and the expression level of vascular endothelial growth factor were higher in the two experimental groups than in the control group. Furthermore, SOD activity was significantly increased (P<0.05), while the neutrophil density and MDA level were significantly reduced (P<0.05) in group II when compared with group I. Significant differences between group II and group III with regard to SOD activity and MDA level were also observed (P<0.05). Thus, DG may have a dose-dependent effect on promoting the survival of random skin flaps.
皮瓣部分坏死仍然限制着整形与重建手术后局部皮瓣的存活。本研究的目的是探究甘草酸二铵（DG），一种已广泛用于慢性肝炎和人类免疫缺陷病毒感染治疗的甘草次酸的盐，对大鼠随意皮瓣存活的影响。将60只雄性Sprague-Dawley大鼠随机分为三组，建立McFarlane皮瓣。第一组作为对照组，每天注射一次生理盐水（10mg/kg）。第二组和第三组为实验组，分别每天注射一次和两次10mg/kg的DG。在第7天，测量皮瓣的存活面积。对组织样本进行苏木精-伊红染色并进行免疫组化评估。评估组织水肿、中性粒细胞密度、超氧化物歧化酶（SOD）活性和丙二醛（MDA）水平。与第一组相比，第二组皮瓣的平均存活面积显著更大（P<0.05），第三组大鼠的存活面积显著高于第二组（P<0.05）。组织学和免疫组化评估显示，两个实验组的微血管发育和血管内皮生长因子的表达水平均高于对照组。此外，与第一组相比，第二组的SOD活性显著增加（P<0.05），而中性粒细胞密度和MDA水平显著降低（P<0.05）。在SOD活性和MDA水平方面，第二组和第三组之间也观察到显著差异（P<0.05）。因此，DG可能对促进随意皮瓣存活具有剂量依赖性作用。
