Li Lin-Yan, Cao Fen-Fang, Su Zhi-Jian, Zhang Qi-Hao, Dai Xiao-Yong, Xiao Xue, Huang Ya-Dong, Zheng Qing, Xu Hua
Department of Microbiological and Biochemical Pharmacy, College of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
Department of Biopharmaceutical Research and Development Centre, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
Mol Med Rep. 2015 Aug;12(2):2348-54. doi: 10.3892/mmr.2015.3598. Epub 2015 Apr 7.
Rhizoma Atractylodes macrocephala, Radix Isatidis, Coptis chinensis and Flos Genkwa are common herbal remedies used by pregnant woman in China. In this study, their potential embryotoxicity was assessed using the embryonic stem cell test (EST) and a prediction model. The potential embryotoxicity of the herbs was based on three endpoints: the concentrations of the compounds that inhibited the proliferation of 50% of embryonic stem cells (ESCs) (IC50ES), the concentrations that inhibited 50% of 3T3 cells (IC503T3), and the concentrations that inhibited the differentiation of 50% of ESCs (ID50ES). The results revealed that Rhizoma Atractylodes macrocephala and Radix Isatidis are non-embryotoxic compounds. Coptis chinensis extracts appeared to demonstrated weak embryotoxicity, and Flos Genkwa exhibited strong embryotoxicity. These results may be useful in guiding the clinical use of these herbs and in expanding the application of the EST to the field of traditional Chinese medicine.
白术、板蓝根、黄连和芫花是中国孕妇常用的草药。在本研究中，使用胚胎干细胞试验（EST）和预测模型评估了它们的潜在胚胎毒性。草药的潜在胚胎毒性基于三个终点：抑制50%胚胎干细胞（ESC）增殖的化合物浓度（IC50ES）、抑制50% 3T3细胞的浓度（IC503T3）以及抑制50% ESC分化的浓度（ID50ES）。结果显示，白术和板蓝根为非胚胎毒性化合物。黄连提取物似乎表现出较弱的胚胎毒性，而芫花表现出较强的胚胎毒性。这些结果可能有助于指导这些草药的临床应用，并将EST的应用扩展到中药领域。
