利用来自产气芽孢杆菌的固定化脂肪酶以非食用蓖麻油生产生物柴油及其特性研究

Narwal Sunil Kumar, Saun Nitin Kumar, Dogra Priyanka, Chauhan Ghanshyam, Gupta Reena

Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, India.

Department of Chemistry, Himachal Pradesh University, Summer Hill, Shimla 171005, India.

Biomed Res Int. 2015;2015:281934. doi: 10.1155/2015/281934. Epub 2015 Mar 22.

A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized. Under the optimized conditions, the highest biodiesel yield was up to 78.13%. The characterization of synthesized biodiesel was done through FTIR spectroscopy, (1)H NMR spectra, and gas chromatography.

一种来自产气芽孢杆菌的新型耐热脂肪酶被固定在廉价的硅胶基质上。固定化脂肪酶用于在化学反应器中于55°C下在无溶剂体系中以蓖麻油为底物合成生物柴油，反应过程中进行振荡。对影响生物柴油产率的几个关键参数，如孵育时间、温度、底物摩尔比和脂肪酶用量进行了优化。在优化条件下，生物柴油的最高产率可达78.13%。通过傅里叶变换红外光谱（FTIR）、核磁共振氢谱（¹H NMR）和气相色谱对合成的生物柴油进行了表征。