Hernández-Flores K G, Vivanco-Cid H
Instituto de Investigaciones Medico-Biológicas, Universidad Veracruzana, 91700 Veracruz, VER, Mexico ; Doctorado en Ciencias Biomédicas, Centro de Investigaciones Biomédicas, Universidad Veracruzana, 91000 Xalapa,VER, Mexico.
Instituto de Investigaciones Medico-Biológicas, Universidad Veracruzana, 91700 Veracruz, VER, Mexico ; Facultad de Medicina "Dr. Porfirio Sosa Zarate", Universidad del Valle de México, Campus Villa Rica, 94299 Boca del Río, VER, Mexico.
Biomed Res Int. 2015;2015:360741. doi: 10.1155/2015/360741. Epub 2015 Mar 22.
Listeriolysin O (LLO) is a thiol-activated cholesterol-dependent pore-forming toxin and the major virulence factor of Listeria monocytogenes (LM). Extensive research in recent years has revealed that LLO exerts a wide array of biological activities, during the infection by LM or by itself as recombinant antigen. The spectrum of biological activities induced by LLO includes cytotoxicity, apoptosis induction, endoplasmic reticulum stress response, modulation of gene expression, intracellular calcium oscillations, and proinflammatory activity. In addition, LLO is a highly immunogenic toxin and the major target for innate and adaptive immune responses in different animal models and humans. Recently, the crystal structure of LLO has been published in detail. Here, we review the structure-function relationship for this fascinating microbial molecule, highlighting the potential uses of LLO in the fields of biomedicine and biotechnology, particularly in vaccination.
溶血素O（LLO）是一种硫醇激活的胆固醇依赖性成孔毒素，也是单核细胞增生李斯特菌（LM）的主要毒力因子。近年来的大量研究表明，在LM感染期间或其作为重组抗原单独存在时，LLO会发挥多种生物学活性。LLO诱导的生物学活性谱包括细胞毒性、凋亡诱导、内质网应激反应、基因表达调节、细胞内钙振荡和促炎活性。此外，LLO是一种高度免疫原性毒素，是不同动物模型和人类固有免疫和适应性免疫反应的主要靶点。最近，LLO的晶体结构已被详细发表。在此，我们综述了这种迷人的微生物分子的结构-功能关系，强调了LLO在生物医学和生物技术领域，特别是在疫苗接种方面的潜在用途。
