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通过生化鉴定被鉴定为 的马匹分离株的分子系统发育分析和基质辅助激光解吸电离飞行时间质谱鉴定 。 你提供的原文似乎不太完整，“identified as by biochemical identification”这里有缺失内容。

Molecular phylogenetic and matrix-assisted laser desorption ionization time-of-flight mass spectrometry identification of isolates from horses identified as by biochemical identification.

作者信息

Uchida-Fujii Eri, Niwa Hidekazu, Kinoshita Yuta, Katayama Yoshinari, Nukada Toshio

机构信息

Microbiology Division, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.

出版信息

J Equine Sci. 2020 Oct;31(3):49-55. doi: 10.1294/jes.31.49. Epub 2020 Oct 5.

DOI:10.1294/jes.31.49
PMID:33061784
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7538261/
Abstract

Enterobacter cloacae is an opportunistic pathogen of horses. Thirty isolates obtained from horses and their environments and identified as Enterobacter cloacae by biochemical methods were reidentified by taxonomic identification based on multilocus sequence analysis (MLSA) and by a commercial identification system based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). MLSA identified the 30 equine isolates as E. ludwigii (9/30), E. asburiae (1/30), or E. cloacae (1/30); 19 isolates were not identified. The MALDI-TOF MS system could not clearly distinguish isolates to the species level, and the limited numbers of reference spectra for Enterobacter species might have contributed to the poor identification.

摘要

阴沟肠杆菌是马的一种机会致病菌。从马及其生活环境中分离得到30株经生化方法鉴定为阴沟肠杆菌的菌株，通过基于多位点序列分析（MLSA）的分类鉴定和基于基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）的商业鉴定系统进行重新鉴定。MLSA将30株马源菌株鉴定为路德维希肠杆菌（9/30）、阿氏肠杆菌（1/30）或阴沟肠杆菌（1/30）；19株未被鉴定。MALDI-TOF MS系统无法在种水平上清晰区分分离株，肠杆菌属参考光谱数量有限可能导致鉴定效果不佳。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e22e/7538261/006ab33b795d/jes-31-049-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e22e/7538261/006ab33b795d/jes-31-049-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e22e/7538261/006ab33b795d/jes-31-049-g001.jpg

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MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.
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MALDI-TOF mass spectrometry for differentiation between Streptococcus pneumoniae and Streptococcus pseudopneumoniae.用于区分肺炎链球菌和伪肺炎链球菌的基质辅助激光解吸电离飞行时间质谱分析
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Identification of bacteria isolated from veterinary clinical specimens using MALDI-TOF MS.使用基质辅助激光解吸电离飞行时间质谱法鉴定从兽医临床样本中分离出的细菌。
Berl Munch Tierarztl Wochenschr. 2015 Jan-Feb;128(1-2):24-30.
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J Microbiol Biotechnol. 2010 Dec;20(12):1614-23. doi: 10.4014/jmb.1005.05014.