Li Chong-Wei, Song Rui-Qing, Yang Li-Bin, Deng Xun
College of Forestry, Northeast Forestry University, Harbin 150040, P.R. China.
Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, P.R. China.
J Microbiol Biotechnol. 2015 Aug;25(8):1257-64. doi: 10.4014/jmb.1410.10027.
Trichoderma strain T-33 has been demonstrated to have inhibitory effect on the fungus species Cytospora chrysosperma. Here, an active antifungal compound was obtained from Trichoderma strain T-33 extract via combined separation technologies, including organic solvent extraction, liquid chromatography, and thin-layer chromatography. The purified compound was further characterized by advanced analytical technologies to elucidate its chemical structure. Results indicated that the active antifungal compound in Trichoderma strain T-33 extract is 2,5- cyclohexadiene-1,4-dione-2,6-bis (1,1-dimethylethyl).
木霉菌株T-33已被证明对真菌种金黄壳囊孢菌有抑制作用。在此，通过包括有机溶剂萃取、液相色谱和薄层色谱在内的联合分离技术，从木霉菌株T-33提取物中获得了一种活性抗真菌化合物。通过先进的分析技术对纯化后的化合物进行进一步表征，以阐明其化学结构。结果表明，木霉菌株T-33提取物中的活性抗真菌化合物是2,5-环己二烯-1,4-二酮-2,6-双（1,1-二甲基乙基）。
