Jaquins-Gerstl Andrea, Michael Adrian C
Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, USA.
Analyst. 2015 Jun 7;140(11):3696-708. doi: 10.1039/c4an02065k.
Microdialysis is commonly used in neuroscience to obtain information about the concentration of substances, including neurotransmitters such as dopamine (DA), in the extracellular space (ECS) of the brain. Measuring DA concentrations in the ECS with in vivo microdialysis and/or voltammetry is a mainstay of investigations into both normal and pathological function of central DA systems. Although both techniques are instrumental in understanding brain chemistry each has its shortcomings. The objective of this review is to characterize some of the tissue and DA differences associated with each technique in vivo. Much of this work will focus on immunohistochemical and microelectrode measurements of DA in the tissue next to the microdialysis probe and mitigating the response to the damage caused by probe implantation.
微透析在神经科学中常用于获取有关大脑细胞外空间（ECS）中物质浓度的信息，这些物质包括多巴胺（DA）等神经递质。通过体内微透析和/或伏安法测量ECS中的DA浓度是研究中枢DA系统正常和病理功能的主要手段。尽管这两种技术在理解大脑化学方面都很重要，但每种技术都有其缺点。本综述的目的是描述体内与每种技术相关的一些组织和DA差异。这项工作的大部分将集中在微透析探针旁边组织中DA的免疫组织化学和微电极测量，以及减轻对探针植入造成的损伤的反应。
