Shadman Niloofar, Ebrahimi Shahram Farzin, Shoul Maryam Azizi, Sattari Hasti
Department of Operative Dentistry, Faculty of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
Postgraduate Student, Department of Operative Dentistry, Faculty of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
Dent Res J (Isfahan). 2015 Mar-Apr;12(2):167-72.
Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) is applied for remineralization of early caries lesions or tooth sensitivity conditions and may affect subsequent resin bonding. This in vitro study investigated the effect of CPP-ACP on the shear bond strength of dental adhesives to enamel.
Sixty extracted human molar teeth were selected and randomly divided into three groups and six subgroups. Buccal or lingual surfaces of teeth were prepared to create a flat enamel surface. Adhesives used were Tetric N-Bond, AdheSE and AdheSE One F. In three subgroups, before applying adhesives, enamel surfaces were treated with Tooth Mousse CPP-ACP for one hour, rinsed and stored in 37°C temperature with 100% humidity. This procedure was repeated for 5 days and then adhesives were applied and Tetric N-Ceram composite was adhered to the enamel. This procedure was also fulfilled for the other three subgroups without CPP-ACP treatment. After 24 hour water storage, samples were tested for shear bond strength test in a universal testing machine. Failure modes were determined by stereomicroscope. Data were analyzed by t-test and one-way analysis of variance with P < 0.05 as the level of significance.
In comparison between applied and non-applied CPP-ACP subgroups, there was no significant decrease in the shear bond strength to enamel only in Tetric N-Bond (P > 0.05). In non-applied CPP-ACP subgroups, there were statistically significant differences among all subgroups. Tetric N-Bond had the highest and AdheSE One F had the lowest shear bond strength.
CPP-ACP application reduces the shear bond strength of AdheSE and AdheSE One F to enamel but not Tetric N-Bond.
酪蛋白磷酸肽 - 无定形磷酸钙（CPP - ACP）用于早期龋损病变或牙齿敏感状况的再矿化，可能会影响后续的树脂粘接。本体外研究调查了CPP - ACP对牙科粘接剂与牙釉质剪切粘接强度的影响。
选取60颗拔除的人类磨牙，随机分为三组和六个亚组。制备牙齿的颊面或舌面以形成平坦的牙釉质表面。使用的粘接剂为Tetric N - Bond、AdheSE和AdheSE One F。在三个亚组中，在应用粘接剂之前，牙釉质表面用Tooth Mousse CPP - ACP处理1小时，冲洗后在37°C温度和100%湿度下储存。此过程重复5天，然后应用粘接剂并将Tetric N - Ceram复合材料粘接至牙釉质。另外三个亚组不进行CPP - ACP处理也完成此过程。在储存24小时水后，在万能试验机上对样品进行剪切粘接强度测试。通过体视显微镜确定失效模式。数据采用t检验和单因素方差分析进行分析，以P < 0.05作为显著性水平。
在应用和未应用CPP - ACP的亚组之间比较，仅Tetric N - Bond对牙釉质的剪切粘接强度没有显著降低（P > 0.05）。在未应用CPP - ACP的亚组中，所有亚组之间存在统计学显著差异。Tetric N - Bond的剪切粘接强度最高，AdheSE One F的最低。
应用CPP - ACP会降低AdheSE和AdheSE One F对牙釉质的剪切粘接强度，但不会降低Tetric N - Bond的。
