Zhang Si-Qi, Li Ping, He Shu-Wen, Xing Shao-Ying, Cao Zhi-Han, Zhao Xue-Li, Sun Cuici, Li Zhi-Hua
Marine College, Shandong University, Weihai, Shandong, 264209, China.
State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 510301, Guangzhou, China.
Environ Sci Ecotechnol. 2023 Mar 23;16:100266. doi: 10.1016/j.ese.2023.100266. eCollection 2023 Oct.
Microplastics (MPs), an emerging group of pollutants, not only have direct toxic effects on aquatic organisms but also cause combined toxicity by absorbing other pollutants. Triphenyltin (TPT), one of the most widely used organotin compounds, has adverse effects on aquatic organisms. However, little is known about the combined toxicity of MPs and TPT to aquatic organisms. To investigate the individual and combined toxicity of MPs and TPT, we selected the common carp () for a 42-day exposure experiment. Based on the environmental concentrations in a heavily polluted area, the experimental concentrations of MPs and TPT were set at 0.5 mg L and 1 μg L, respectively. The effects of MPs combined with TPT on the carp gut-brain axis were evaluated by detecting gut physiology and biochemical parameters, gut microbial 16S rRNA, and brain transcriptome sequencing. Our results suggest that a single TPT caused lipid metabolism disorder and a single MP induced immunosuppression in carp. When MPs were combined with TPT, the involvement of TPT amplified the immunotoxic effect induced by MPs. In this study, we also explored the gut-brain axis relationship of carp immunosuppression, providing new insights for assessing the combined toxicity of MPs and TPT. At the same time, our study provides a theoretical basis for evaluating the coexistence risk of MPs and TPT in the aquatic environment.
微塑料（MPs）作为一类新出现的污染物，不仅对水生生物具有直接毒性作用，还会通过吸附其他污染物产生联合毒性。三苯基锡（TPT）是使用最为广泛的有机锡化合物之一，对水生生物具有不良影响。然而，关于微塑料和三苯基锡对水生生物的联合毒性却知之甚少。为了探究微塑料和三苯基锡的单一及联合毒性，我们选用鲤鱼（ ）进行了为期42天的暴露实验。基于污染严重区域的环境浓度，将微塑料和三苯基锡的实验浓度分别设定为0.5毫克/升和1微克/升。通过检测肠道生理生化参数、肠道微生物16S rRNA以及脑转录组测序，评估微塑料与三苯基锡联合作用对鲤鱼肠道-脑轴的影响。我们的研究结果表明，单一的三苯基锡会导致鲤鱼脂质代谢紊乱，单一的微塑料会诱导鲤鱼免疫抑制。当微塑料与三苯基锡联合时，三苯基锡的参与放大了微塑料诱导的免疫毒性效应。在本研究中，我们还探究了鲤鱼免疫抑制的肠道-脑轴关系，为评估微塑料和三苯基锡的联合毒性提供了新的见解。同时，我们的研究为评估微塑料和三苯基锡在水生环境中的共存风险提供了理论依据。
