环丙沙星对快速生长分枝杆菌形成生物膜的超微结构及发育的影响。
Effect of ciprofloxacin in the ultrastructure and development of biofilms formed by rapidly growing mycobacteria.
作者信息
Muñoz-Egea María-Carmen, García-Pedrazuela María, Mahillo Ignacio, Esteban Jaime
机构信息
Department of Clinical Microbiology, IIS- Fundación Jiménez Díaz. Av. Reyes Católicos 2, 28040, Madrid, Spain.
Department of Epidemiology, IIS- Fundación Jiménez Díaz Av. Reyes Católicos 2, 28040, Madrid, Spain.
出版信息
BMC Microbiol. 2015 Feb 4;15:18. doi: 10.1186/s12866-015-0359-y.
BACKGROUND
The aim of this study was to analyze the effect of ciprofloxacin at different times on the development and behavior of intrinsic autofluorescence, covered area, thickness and cell viability in a biofilm formed by non-pigmented rapidly growing mycobacteria (NPRGM).Confocal laser scanning microscopy and image analysis were used to study the behavior of ciprofloxacin on biofilms.
RESULTS
Thickness was the most affected parameter, although some species showed changes in other parameters. At the same time, we also measured the minimum inhibitory concentration and the minimum biofilm eradication concentration (MBEC). An increase in MBEC was observed in all the strains, M. peregrinum being the species that presented the highest increase.
CONCLUSIONS
This study help us to understand better how mycobacterial biofims can be affected by ciprofloxacin.
背景
本研究旨在分析不同时间的环丙沙星对非色素快速生长分枝杆菌（NPRGM）形成的生物膜中固有自发荧光的发展和行为、覆盖面积、厚度及细胞活力的影响。采用共聚焦激光扫描显微镜和图像分析来研究环丙沙星对生物膜的作用。
结果
厚度是受影响最大的参数，不过某些菌株在其他参数上也有变化。同时，我们还测定了最低抑菌浓度和最低生物膜清除浓度（MBEC）。所有菌株的MBEC均有升高，其中外来分枝杆菌升高幅度最大。
结论
本研究有助于我们更好地理解环丙沙星如何影响分枝杆菌生物膜。
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