Terentieva Daria, Savova-Lalkovska Tanya, Dimitrova Albena, Bonovska Magdalena, Mokrousov Igor, Valcheva Violeta
Laboratory of Molecular Epidemiology and Evolutionary Genetics, St. Petersburg Pasteur Institute, 197101 St. Petersburg, Russia.
National Reference Laboratory for Bovine Tuberculosis, National Diagnostic and Research Veterinary Medical Institute "Prof. Dr. G. Pavlov", 1606 Sofia, Bulgaria.
Diagnostics (Basel). 2023 Feb 17;13(4):771. doi: 10.3390/diagnostics13040771.
The aim of this study was to assess the diversity of minisatellite VNTR loci in / isolates in Bulgaria and view their position within global diversity. Forty-three / isolates from cattle in different farms in Bulgaria were collected in 2015-2021 and typed in 13 VNTR loci. The and branches were clearly separated on the VNTR phylogenetic tree. The larger and more geographically dispersed group was more diverse than group was (HGI 0.67 vs. 0.60). Overall, six clusters were identified (from 2 to 19 isolates) and nine orphans (all loci-based HGI 0.79). Locus QUB3232 was the most discriminatory one (HGI 0.64). MIRU4 and MIRU40 were monomorphic, and MIRU26 was almost monomorphic. Four loci (ETRA, ETRB, Mtub21, and MIRU16) discriminated only between and . The comparison with published VNTR datasets from 11 countries showed both overall heterogeneity between the settings and predominantly local evolution of the clonal complexes. To conclude, six loci may be recommended for primary genotyping of / isolates in Bulgaria: ETRC, QUB11b, QUB11a, QUB26, QUB3232, and MIRU10 (HGI 0.77). VNTR typing based on a limited number of loci appears to be useful for primary bTB surveillance.
本研究的目的是评估保加利亚/分离株中微卫星VNTR基因座的多样性，并观察它们在全球多样性中的位置。2015年至2021年期间，从保加利亚不同农场的牛身上收集了43株/分离株，并对13个VNTR基因座进行了分型。在VNTR系统发育树上，和分支明显分开。规模更大且地理分布更广的组比组更具多样性（HGI分别为0.67和0.60）。总体而言，共鉴定出六个聚类（包含2至19个分离株）和九个孤株（基于所有基因座的HGI为0.79）。基因座QUB3232的鉴别力最强（HGI为0.64）。MIRU4和MIRU40呈单态性，MIRU26几乎呈单态性。四个基因座（ETRA、ETRB、Mtub21和MIRU16）仅能区分和。与来自11个国家的已发表VNTR数据集进行比较，结果显示不同环境之间总体存在异质性，且克隆复合体主要是局部进化。总之，对于保加利亚/分离株的初步基因分型，推荐使用六个基因座：ETRC、QUB11b、QUB11a、QUB26、QUB3232和MIRU10（HGI为0.77）。基于有限数量基因座的VNTR分型似乎对原发性牛结核病监测有用。
