Kawano Y
Nihon Ketsueki Gakkai Zasshi. 1989 Aug;52(5):831-4.
PHA-leukocytes conditioned medium (PHA-LCM) prepared from the patients with chronic renal failure and normal control was tested for BPA by progenitor cell assay with methylcellulose culture method. The BPA of uremic patients, expressed as a percentage of standard CM, was significantly lower than that of normal subjects (96 +/- 9%). However, there was no correlation between BPA and the severity of anemia. The number of circulating BFU-E per milliliter of blood was significantly lower in uremic patients (71 +/- 77) than in normal controls (131 +/- 96), and the number correlated with the severity of anemia. From these results, the maturation process of erythroid series in uremic patients appeared to be impaired at a stage between pluripotent stem cell and BFU-E, and might be secondary to inefficient production of BPA by lymphocytes.
采用甲基纤维素培养法，通过祖细胞测定法对慢性肾衰竭患者和正常对照者制备的PHA-白细胞条件培养基（PHA-LCM）进行爆式集落形成单位（BPA）检测。以标准条件培养基的百分比表示，尿毒症患者的BPA显著低于正常受试者（96±9%）。然而，BPA与贫血严重程度之间无相关性。尿毒症患者每毫升血液中循环爆式红系集落形成单位（BFU-E）的数量（71±77）显著低于正常对照者（131±96），且该数量与贫血严重程度相关。根据这些结果，尿毒症患者红系的成熟过程似乎在多能干细胞和BFU-E之间的阶段受损，可能继发于淋巴细胞产生BPA效率低下。
