Righolt Christiaan H, Schmälter Ann-Kristin, Kuzyk Alexandra, Young Ian T, van Vliet Lucas J, Mai Sabine
Manitoba Institute of Cell Biology, CancerCare Manitoba, University of Manitoba, Winnipeg, Manitoba, Canada.
Department of Imaging Physics, Delft University of Technology, Delft, The Netherlands.
Cytometry A. 2015 Aug;87(8):733-40. doi: 10.1002/cyto.a.22674. Epub 2015 Apr 17.
The nuclear architecture of a cell may change as a result of various diseases, including cancer. A variety of nuclear features are, therefore, of interest to cell biologists. Recently, several studies have investigated the orientation of chromosomes in the interphase nucleus either visually or semi-automatically. In this article an automated method to measure this orientation is presented. The theoretical difference between performing these measurements in two and three dimensions is discussed and experimentally verified. The results computed from measurements of murine nuclei correspond with results from visual inspection. We found significant differences in the orientation of chromosome 11 between nuclei from a PreB cell line of BALB/c origin and primary B nuclei from congenic [T38HxBALB/c]N wild-type mice. Since our new automatic method concurs with both the visual and semi-automatic methods, we conclude that the automatic method can replace these methods in assessing chromosome orientation.
细胞的核结构可能会因包括癌症在内的各种疾病而发生变化。因此，细胞生物学家对多种核特征感兴趣。最近，有几项研究通过视觉或半自动方式研究了间期细胞核中染色体的取向。本文提出了一种测量这种取向的自动化方法。讨论并通过实验验证了在二维和三维中进行这些测量的理论差异。从小鼠细胞核测量计算得到的结果与目视检查结果相符。我们发现，源自BALB/c的PreB细胞系的细胞核与同基因[T38HxBALB/c]N野生型小鼠的原代B细胞核中，11号染色体的取向存在显著差异。由于我们的新自动化方法与视觉和半自动方法一致，我们得出结论，在评估染色体取向时，自动化方法可以取代这些方法。
