Amal B, Veena B, Jayachandran V P, Shilpa Joy
Department of Biotechnology, C.M.S College, Kottayam, Kerala, India.
J Mater Sci Mater Med. 2015 May;26(5):181. doi: 10.1007/s10856-015-5515-2. Epub 2015 Apr 17.
Engineered scaffolds made from natural biomaterials are crucial elements in tissue engineering strategies. In this study, biological scaffold like chitosan-collagen-starch membrane (CCSM) loaded with the antibacterial agent, Punica granatum pericarp aqueous extract was explored for enhanced regeneration of epithelial tissue during wound healing. Collagen was extracted from Rachycentron canadum fish skin. Membranous scaffold was prepared by mixing collagen, starch and chitosan in a fixed proportion, loaded with aqueous extract of P. granatum and its anti-pseudomonal activity was studied. Morphological characterization by SEM and mechanical property like tensile strength of the membrane were studied. Excision wound of 2 cm(2) size was induced in Guinea pig and the effect of P. granatum extract loaded CCSM in wound healing was studied. The SEM image showed deep pores in the membrane and also possessed good tensile strength. Wound surface area was reduced prominently in the experimental group with P. granatum extract loaded CCSM when compared to the group with unloaded membrane and the one with no membrane. Punica granatum extract loaded CCSM has antipseudomonal property and supported enhanced epithelial cell proliferation without leaving a scar after wound healing. This has significant therapeutic application in membranous scaffold mediated skin repair and regeneration.
由天然生物材料制成的工程支架是组织工程策略中的关键要素。在本研究中，探索了负载抗菌剂石榴果皮水提取物的壳聚糖 - 胶原蛋白 - 淀粉膜（CCSM）这种生物支架，以促进伤口愈合过程中上皮组织的再生。胶原蛋白从军曹鱼鱼皮中提取。通过按固定比例混合胶原蛋白、淀粉和壳聚糖制备膜状支架，负载石榴皮水提取物并研究其抗假单胞菌活性。研究了通过扫描电子显微镜（SEM）进行的形态表征以及膜的拉伸强度等力学性能。在豚鼠身上制造2平方厘米大小的切除伤口，并研究负载石榴皮提取物的CCSM在伤口愈合中的作用。扫描电子显微镜图像显示膜中有深孔，并且具有良好的拉伸强度。与未负载膜的组和无膜组相比，负载石榴皮提取物的CCSM实验组的伤口表面积显著减小。负载石榴皮提取物的CCSM具有抗假单胞菌特性，并支持上皮细胞增殖增强，伤口愈合后不留疤痕。这在膜状支架介导的皮肤修复和再生中具有重要的治疗应用。
