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Targeted gene disruption in Xenopus laevis using CRISPR/Cas9.

作者信息

Wang Fengqin, Shi Zhaoying, Cui Yan, Guo Xiaogang, Shi Yun-Bo, Chen Yonglong

机构信息

School of Life Sciences, Anhui University, Hefei, 230601 China ; CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.

Department of Biology, Shenzhen Key Laboratory of Cell Microenvironment, South University of Science and Technology of China, Shenzhen, 518055 China.

出版信息

Cell Biosci. 2015 Apr 14;5:15. doi: 10.1186/s13578-015-0006-1. eCollection 2015.

DOI:10.1186/s13578-015-0006-1
PMID:25897376
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4403895/
Abstract
摘要

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Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.反向遗传学筛选揭示了斑马鱼中 morpholino 诱导表型和突变体表型之间的相关性较差。
Dev Cell. 2015 Jan 12;32(1):97-108. doi: 10.1016/j.devcel.2014.11.018. Epub 2014 Dec 18.
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Engineering Xenopus embryos for phenotypic drug discovery screening.利用爪蟾胚胎进行表型药物发现筛选的工程技术。
Adv Drug Deliv Rev. 2014 Apr;69-70:225-46. doi: 10.1016/j.addr.2014.02.004. Epub 2014 Feb 24.
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Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis.
BMC Genomics. 2024 Nov 1;25(1):1025. doi: 10.1186/s12864-024-10899-7.
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The Amphibian Genomics Consortium: advancing genomic and genetic resources for amphibian research and conservation.两栖动物基因组学联盟：推进两栖动物研究与保护的基因组和遗传资源。
bioRxiv. 2024 Oct 3:2024.06.27.601086. doi: 10.1101/2024.06.27.601086.
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Intestinal remodeling during Xenopus metamorphosis as a model for studying thyroid hormone signaling and adult organogenesis.在爪蟾变态过程中肠道重塑作为研究甲状腺激素信号和成年器官发生的模型。
Mol Cell Endocrinol. 2024 May 15;586:112193. doi: 10.1016/j.mce.2024.112193. Epub 2024 Feb 22.
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Steroid-receptor coactivator complexes in thyroid hormone-regulation of Xenopus metamorphosis.类固醇受体共激活因子复合物在甲状腺激素调控非洲爪蟾变态中的作用。
Vitam Horm. 2023;123:483-502. doi: 10.1016/bs.vh.2023.02.003. Epub 2023 Mar 9.
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Breaking Through the Bottleneck: Krogh's Principle in Behavioral Neuroendocrinology and the Potential of Gene Editing.突破瓶颈：Krogh 原理在行为神经内分泌学中的应用及基因编辑的潜力。
Integr Comp Biol. 2023 Aug 23;63(2):428-443. doi: 10.1093/icb/icad068.
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Cell cycle activation in thyroid hormone-induced apoptosis and stem cell development during intestinal metamorphosis.甲状腺激素诱导的细胞凋亡和肠变态过程中干细胞发育的细胞周期激活。
Front Endocrinol (Lausanne). 2023 May 17;14:1184013. doi: 10.3389/fendo.2023.1184013. eCollection 2023.
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Review: Recent Applications of Gene Editing in Fish Species and Aquatic Medicine.综述：基因编辑在鱼类物种和水产医学中的最新应用
Animals (Basel). 2023 Apr 4;13(7):1250. doi: 10.3390/ani13071250.
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Genetic dissection of steroid-hormone modulated social behavior: Novel paralogous genes are a boon for discovery.类固醇激素调节的社会行为的遗传剖析：新的同源基因是发现的福音。
Horm Behav. 2023 Jan;147:105295. doi: 10.1016/j.yhbeh.2022.105295. Epub 2022 Dec 8.
高效的 RNA/Cas9 介导的 Xenopus tropicalis 基因组编辑。
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4
Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases.利用两对转录激活样效应核酸酶对非洲爪蟾中多个同源基因进行靶向诱变。
Dev Growth Differ. 2014 Jan;56(1):108-14. doi: 10.1111/dgd.12105. Epub 2013 Dec 11.
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High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos.高效 TALENs 通过靶向基因敲除在非洲爪蟾胚胎中实现 F0 功能分析。
Biol Open. 2013 Mar 4;2(5):448-52. doi: 10.1242/bio.20133855. Print 2013 May 15.
6
Generation of gene disruptions by transcription activator-like effector nucleases (TALENs) in Xenopus tropicalis embryos.通过转录激活子样效应物核酸酶（TALENs）在非洲爪蟾胚胎中产生基因缺失。
Cell Biosci. 2013 May 10;3(1):21. doi: 10.1186/2045-3701-3-21.
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Controlling morpholino experiments: don't stop making antisense.控制吗啉代寡核苷酸实验：不要停止制备反义寡核苷酸。
Development. 2008 May;135(10):1735-43. doi: 10.1242/dev.001115. Epub 2008 Apr 9.
8
Combined ectopic expression of Pdx1 and Ptf1a/p48 results in the stable conversion of posterior endoderm into endocrine and exocrine pancreatic tissue.Pdx1与Ptf1a/p48的联合异位表达导致后端内胚层稳定转化为内分泌和外分泌胰腺组织。
Genes Dev. 2006 Jun 1;20(11):1441-6. doi: 10.1101/gad.378706.
9
The bHLH protein PTF1-p48 is essential for the formation of the exocrine and the correct spatial organization of the endocrine pancreas.bHLH蛋白PTF1-p48对于外分泌腺的形成以及内分泌胰腺的正确空间组织至关重要。
Genes Dev. 1998 Dec 1;12(23):3752-63. doi: 10.1101/gad.12.23.3752.