Vaz-Tostes Maria das Graças, Verediano Thaisa Agrizzi, de Mejia Elvira Gonzalez, Brunoro Costa Neuza Maria
Department of Pharmacy and Nutrition, Center for Agricultural Sciences, Federal University of Espirito Santo, Alto Universitario, 29500-000, Alegre, ES, Brazil.
Department of Nutrition and Health, Federal University of Viçosa, PH Holfs, 36570-000, Viçosa, MG, Brazil.
J Sci Food Agric. 2016 Mar 15;96(4):1326-32. doi: 10.1002/jsfa.7226. Epub 2015 May 22.
Biofortified beans have been produced with higher nutrient concentrations. The objective was to evaluate the in vitro and in vivo iron and zinc bioavailability of common beans Pontal (PO), targeted for biofortification, compared with conventional Perola (PE) and their effects on the iron and zinc nutritional status of preschool children.
In Caco-2 cells, PO and PE beans did not show differences in ferritin (PO, 13.1 ± 1.4; PE, 13.6 ± 1.4 ng mg(-1) protein) or zinc uptake (PO, 15.9 ± 1.5; PE, 15.5 ± 3.5 µmol mg(-1) protein). In the rat, PO and PE beans presented high iron bioavailability (PO, 109.6 ± 29.5; PE, 110.7 ± 13.9%). In preschool children, no changes were observed in iron and zinc nutritional status comparing before and after PO consumption (ferritin, 41.2 ± 23.2 and 28.9 ± 40.4 µg L(-1) ; hemoglobin, 13.7 ± 2.2 and 13.1 ± 3.2 g dL(-1) ; plasma zinc, 119.2 ± 24.5 and 133.9 ± 57.7 µg dL(-1) ; erythrocyte zinc, 53.5 ± 13.8 and 59.4 ± 17.1 µg g(-1) hemoglobin).
Iron and zinc bioavailability in PO and PE beans was not statistically different using either cell culture, animal or human models. Efforts should focus on increasing mineral bioavailability of beans targeted for biofortification.
已培育出营养成分浓度更高的生物强化豆类。目的是评估旨在进行生物强化的蓬塔尔普通豆（PO）与传统的佩罗拉豆（PE）在体外和体内的铁和锌生物利用度，以及它们对学龄前儿童铁和锌营养状况的影响。
在Caco-2细胞中，PO豆和PE豆在铁蛋白（PO，13.1±1.4；PE，13.6±1.4 ng mg⁻¹蛋白质）或锌摄取（PO，15.9±1.5；PE，15.5±3.5 μmol mg⁻¹蛋白质）方面没有差异。在大鼠中，PO豆和PE豆呈现出高铁生物利用度（PO，109.6±29.5；PE，110.7±13.9%）。在学龄前儿童中，比较食用PO豆前后，铁和锌营养状况未观察到变化（铁蛋白，41.2±23.2和28.9±40.4 μg L⁻¹；血红蛋白，13.7±2.2和13.1±3.2 g dL⁻¹；血浆锌，119.2±24.5和133.9±57.7 μg dL⁻¹；红细胞锌，53.5±13.8和59.4±17.1 μg g⁻¹血红蛋白）。
使用细胞培养、动物或人体模型，PO豆和PE豆中的铁和锌生物利用度在统计学上没有差异。应致力于提高旨在进行生物强化的豆类的矿物质生物利用度。
