Nguyen Tuan Manh, Kim Jaisoo
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 443-760, Republic of Korea.
Int J Syst Evol Microbiol. 2015 Aug;65(8):2385-2390. doi: 10.1099/ijs.0.000268. Epub 2015 Apr 21.
Strain T258T was isolated from forest soil at Bongnae Falls, South Korea. The strain exhibited antimicrobial and antifungal activity against the following strains: Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Paenibacillus larvae, Escherichia coli, Candida albicans and Aspergillus niger. Growth occurred on all ISP media tested (2, 3, 4, 5, 6 and 7), Czapek-Dox agar, potato dextrose agar, trypticase soy agar, Bennett's modified agar and nutrient agar at 28 °C. Aerial spores were produced solely on ISP Medium 4; the colour of the aerial mycelium was white and the substrate mycelium was ivory. Melanin production was negative on peptone-yeast extract iron agar (ISP Medium 6). The cell-wall peptidoglycan contained ll-diaminopimelic acid, glutamic acid, alanine and glycine. Whole-cell hydrolysates contained glucose, ribose and galactose. The predominant menaquinones were MK-9(H6) and MK-9(H8) while the minor menaquinone was MK-10(H2). The polar lipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids (>10%) were C16 : 0 (29.8%), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (15.1%), anteiso-C15 : 0 (13.5%) and iso-C15 : 0 (10.3%). DNA-DNA similarity with other strains ranged between 37.84 ± 1.15% and 50.25 ± 1.91 %. On the basis of these data, we suggest that strain T258T represents a novel species that belong to the genus Streptomyces, for which we propose a name Streptomyces polymachus sp. nov. The type strain is T258T ( = KACC 18247T = KEMB 9005-212T = NBRC 110905T).
T258T菌株是从韩国凤来瀑布的森林土壤中分离出来的。该菌株对以下菌株表现出抗菌和抗真菌活性：枯草芽孢杆菌、金黄色葡萄球菌、铜绿假单胞菌、幼虫类芽孢杆菌、大肠杆菌、白色念珠菌和黑曲霉。在所有测试的ISP培养基（2、3、4、5、6和7）、查氏琼脂、马铃薯葡萄糖琼脂、胰蛋白胨大豆琼脂、贝内特改良琼脂和营养琼脂上，于28°C时均能生长。仅在ISP培养基4上产生气生孢子；气生菌丝的颜色为白色，基内菌丝为象牙色。在蛋白胨酵母提取物铁琼脂（ISP培养基6）上黑色素产生呈阴性。细胞壁肽聚糖含有ll-二氨基庚二酸、谷氨酸、丙氨酸和甘氨酸。全细胞水解产物含有葡萄糖、核糖和半乳糖。主要的甲基萘醌是MK-9(H6)和MK-9(H8)，次要的甲基萘醌是MK-10(H2)。极性脂质包括双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇。主要脂肪酸（>10%）为C16 : 0（29.8%）、总和特征3（C16 : 1ω7c和/或C16 : 1ω6c）（15.1%）、anteiso-C15 : 0（13.5%）和iso-C15 : 0（10.3%）。与其他菌株的DNA-DNA相似性在37.84 ± 1.15%至50.25 ± 1.91 %之间。基于这些数据，我们认为T258T菌株代表链霉菌属的一个新物种，为此我们提议命名为多战链霉菌（Streptomyces polymachus sp. nov.）。模式菌株为T258T（=KACC 18247T = KEMB 9005-212T = NBRC 110905T）。
