Zhang Binglin, Tang Shukun, Chen Ximing, Zhang Ling, Zhang Gaoseng, Zhang Wei, Liu Guangxiu, Chen Tuo, Li Shiweng, Dyson Paul
State Key Laboratory of Cryospheric Sciences, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy Sciences, Lanzhou 730000, PR China.
Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province 730000, PR China.
Int J Syst Evol Microbiol. 2016 Dec;66(12):4981-4986. doi: 10.1099/ijsem.0.001456. Epub 2016 Sep 5.
A novel actinobacterial strain, designated Z1027T, was isolated from a soil sample collected near the Tuotuo River, Qinghai-Tibet Plateau (China). The strain exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus. The taxonomic position of strain Z1027T was determined using a polyphasic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Streptomyces and formed a distinct phyletic line in the 16S rRNA gene tree, together with Streptomyces turgidiscabies ATCC 700248T (99.19 % similarity), Streptomyces graminilatus JL-6T (98.84 %) and Streptomyces reticuliscabiei CFBP 4531T (98.36 %). The genomic DNA G+C content of strain Z1027T was 74±1 mol%. The DNA-DNA relatedness values between strain Z1027T and Streptomyces turgidiscabies ATCC 700248T and Streptomyces reticuliscabiei CFBP 4531T were 38.5±0.4 and 26.2±1.2 %, respectively, both of them significantly lower than 70 %. Chemotaxonomic data revealed that strain Z1027T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, ll-diaminopimelic acid as the diagnostic diamino acid and galactose as a whole-cell sugar. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatydilinositol and seven other unknown polar lipids were detected; iso-C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0 were the major fatty acids. On the basis of these genotypic and phenotypic data, it is proposed that isolate Z1027T (=CGMCC 4.7272T=JCM 31054T) should be classified as the type strain of a novel species of the genus Streptomyces,Streptomyces lacrimifluminis sp. nov.
从中国青藏高原沱沱河附近采集的土壤样本中分离出一株新型放线菌，命名为Z1027T。该菌株对大肠杆菌和金黄色葡萄球菌具有抗菌活性。采用多相分类法确定了菌株Z1027T的分类地位。该菌株的化学分类和形态学特征与其在链霉菌属中的分类一致，在16S rRNA基因树中与肿胀链霉菌ATCC 700248T（相似度99.19%）、禾本科链霉菌JL-6T（98.84%）和网状链霉菌CFBP 4531T（98.36%）形成一个独特的进化分支。菌株Z1027T的基因组DNA G+C含量为74±1 mol%。菌株Z1027T与肿胀链霉菌ATCC 700248T和网状链霉菌CFBP 4531T之间的DNA-DNA杂交值分别为38.5±0.4%和26.2±1.2%，均显著低于70%。化学分类数据表明，菌株Z1027T以MK-9(H6)和MK-9(H8)作为主要甲基萘醌，ll-二氨基庚二酸作为特征性二氨基酸，半乳糖作为全细胞糖。检测到双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇和其他七种未知极性脂质；异-C16 : 0、特征性总和3（C16 : 1ω7c和/或C16 : 1ω6c）和C16 : 0为主要脂肪酸。基于这些基因型和表型数据，建议将分离株Z1027T（=CGMCC 4.7272T=JCM 31054T）分类为链霉菌属一个新物种的模式菌株，即泪河链霉菌新种（Streptomyces lacrimifluminis sp. nov.）。
