Sharma Kalpesh K, Ahmed Faruq, Schenk Peer M, Li Yan
Algae Biotechnology Laboratory, School of Agriculture and Food Sciences, The University of Queensland, Brisbane, Queensland, 4072, Australia.
College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, 4811, Australia.
Biotechnol Bioeng. 2015 Oct;112(10):2106-14. doi: 10.1002/bit.25621. Epub 2015 Jul 22.
Microalgae are primary producers of organic pigments carotenoids in aquatic environments. However, commercial-scale microalgae application for high value carotenoids production is rarely economical due to the cost-effectiveness of carotenoid induction and microalgal harvesting process. Here, we present a novel approach, using a small dose of externally applied UV-C radiation, to rapidly induce unsaturated fatty acids and carotenoid biosynthesis in Dunaliella salina and Haematococcus pluvialis, and also to significantly promote their swimming cells settling for primary dewatering. The amount of total carotenoids and β-carotenoid were doubled in 24 h on D. salina upon 50 mJ/cm(2) of UV-C radiation, whereas the astaxanthin yield of H. pluvialis was increased five times in 48 h at 30 mJ/cm(2) . Meanwhile, 95% of algal cells of D. salina and H. pluvialis settled in 15 h and 2 h, respectively. This novel technique represents a convenient, time-saving and cost-effective method for commercial microalgal carotenoids production.
微藻是水生环境中有机色素类胡萝卜素的主要生产者。然而，由于类胡萝卜素诱导和微藻收获过程的成本效益，商业规模的微藻用于生产高价值类胡萝卜素的应用很少具有经济性。在此，我们提出一种新方法，即使用小剂量外部施加的UV-C辐射，来快速诱导盐藻和雨生红球藻中不饱和脂肪酸和类胡萝卜素的生物合成，并且显著促进它们游动细胞的沉降以进行初次脱水。在50 mJ/cm(2) 的UV-C辐射下，盐藻中的总类胡萝卜素和β-胡萝卜素含量在24小时内翻倍，而在30 mJ/cm(2) 时，雨生红球藻的虾青素产量在48小时内增加了五倍。同时，盐藻和雨生红球藻分别有95%的藻细胞在15小时和2小时内沉降。这项新技术代表了一种用于商业微藻类胡萝卜素生产的便捷、省时且具有成本效益的方法。
