Gulcelik Mehmet A, Dincer Halil, Sahin Duygu, Faruk Demir Omer, Yenidogan Erdinc, Alagol Haluk
Ankara Oncology Training and Research Hospital, Ankara, Turkey; Email:
Wounds. 2010 Jan;22(1):12-6.
Purpose. Diabetes mellitus (DM) is a contributing factor to impaired wound healing in humans. A large body of evidence indicates that the diabetic state is associated with delayed or reduced wound repair capacity. The present study was designed to evaluate the efficacy of glucan on improving abdominal wall wound healing in rats with DM.
Ninety-six female, Sprague-Dawley rats that weighed between 250 g and 300 g were used. A laparotomy was performed on all of the rats on the 14th day. Twenty-four healthy rats (group 1) served as the control. Streptozotocin was used to induce DM in groups 2 and 3 (n = 48). Rats in group 3 received glucan (n = 24). Rats in group 4 were not rendered with DM but received glucan (n = 24). The sutures were removed and abdominal bursting pressure was measured and recorded on the seventh postoperative day for all of the groups. Tissue samples were taken from the incision line for histopathological evaluation and hydroxyproline measurement.
In group 2, the bursting pressure was significantly lower than in groups 1, 3, and 4; the hydroxyproline content and histopathological evaluations also supported these findings.
These results demonstrate that glucan improves impaired wound healing in rats with DM. .
目的。糖尿病（DM）是导致人类伤口愈合受损的一个因素。大量证据表明，糖尿病状态与伤口修复能力延迟或降低有关。本研究旨在评估葡聚糖对改善糖尿病大鼠腹壁伤口愈合的疗效。
使用96只体重在250克至300克之间的雌性Sprague-Dawley大鼠。在第14天对所有大鼠进行剖腹手术。24只健康大鼠（第1组）作为对照。用链脲佐菌素在第2组和第3组（n = 48）诱导糖尿病。第3组大鼠接受葡聚糖（n = 24）。第4组大鼠未诱导糖尿病但接受葡聚糖（n = 24）。在术后第七天对所有组的大鼠拆除缝线并测量和记录腹壁破裂压力。从切口线取组织样本进行组织病理学评估和羟脯氨酸测量。
在第2组中，破裂压力显著低于第1组、第3组和第4组；羟脯氨酸含量和组织病理学评估也支持这些结果。
这些结果表明葡聚糖可改善糖尿病大鼠受损的伤口愈合。
