Kamer Erdinc, Recai Unalp Haluk, Gundogan Omer, Diniz Gulden, Ortac Ragip, Olukman Murat, Derici Hayrullah, Ali Onal Mehmet
Department of Surgery, Izmir Ataturk Training and Research Hospital, Izmir, Turkery; Email:
Wounds. 2010 Feb;22(2):27-31.
Abstract: Background. Abdominal wall repair after celiotomy is important because insufficient incisional wound strength results in wound failures such as fascial dehiscence and herniation. Ascorbic acid has been shown to play an important role in wound healing. The purpose of this study was to investigate whether ascorbic acid improves incisional wound healing in a diabetic rat.
Male Wistar-Albino streptozosin-induced diabetic rats (n = 20) were divided into two groups: control group (CG; n = 10), and daily 200 mg/kg ascorbic acid (study group, [SG], n = 10) given orally. Ten animals from each group were euthanized on postoperative day (POD) 14 after wounding; breaking strength, histologic examination, and tissue hydroxyproline levels were analyzed.
The hydroxyproline tissue content of the abdominal fascia in the ascorbic acid treatment group was superior to the control group, and the difference was statistically significant (P < 0.05). The tensiometric analyses revealed that tensile strength for the midline incision was significantly higher in the study group compared to the control group (P < 0.05). Significant differences were found in the results of histologic examination of tissue specimens between the two groups regarding acute inflammation, chronic inflammation, granulation tissue fibroblast maturation, collagen deposition, and neovascularization on POD 14 (P < 0.05).
The present study demonstrates that administration of ascorbic acid prior to laparotomy expedites wound healing in a rat. On the contrary, we suggest that it could confer benefits to tissue healing by significantly enhancing tissue hydroxyproline levels, neovascularization, fibroblast maturation, and collagen deposition.
摘要：背景。剖腹术后腹壁修复很重要，因为切口伤口强度不足会导致伤口愈合不良，如筋膜裂开和疝形成。已证明维生素C在伤口愈合中起重要作用。本研究的目的是调查维生素C是否能改善糖尿病大鼠的切口伤口愈合。
雄性Wistar - 白化链脲佐菌素诱导的糖尿病大鼠（n = 20）分为两组：对照组（CG；n = 10）和每日口服200 mg/kg维生素C的组（研究组，[SG]，n = 10）。每组10只动物在受伤后第14天处死；分析断裂强度、组织学检查和组织羟脯氨酸水平。
维生素C治疗组腹部筋膜的羟脯氨酸组织含量高于对照组，差异有统计学意义（P < 0.05）。张力分析显示，研究组中线切口的抗张强度明显高于对照组（P < 0.05）。两组在术后第14天组织标本的组织学检查结果在急性炎症、慢性炎症、肉芽组织成纤维细胞成熟、胶原沉积和新生血管形成方面存在显著差异（P < 0.05）。
本研究表明，剖腹术前给予维生素C可加速大鼠伤口愈合。相反，我们认为它可以通过显著提高组织羟脯氨酸水平、新生血管形成、成纤维细胞成熟和胶原沉积来促进组织愈合。
