Abedini Fateme, Mohammadi Shahla Roudbar, Dahmardehei Mostafa, Ajami Marjan, Salimi Maryam, Khalandi Halala, Mohsenzadegan Monireh, Seif Farhad, Shirvan Bahador Nikoueian, Yaalimadad Sanaz, Roudbary Maryam, Rodrigues Célia F
Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran 1449614535, Iran.
Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 14115-111, Iran.
J Fungi (Basel). 2022 Mar 4;8(3):263. doi: 10.3390/jof8030263.
The mortality and disability-adjusted life years (DALYs) of burn patients are decreasing over time. However, finding novel effective treatment approaches using natural agents is highly considered to reduce the burden of burn injuries. One of the recent agents used in wound healing is β-glucan, mainly extracted from fungi cell walls. This study aimed to evaluate the effect of 5% (m/m) of yeast β-glucan ointment on burn wound healing and to assess the impact of β-glucan on cytokines during the treatment. Thirty-three patients with second or third-degree burns were enrolled in this study. Two groups of twenty-three and ten patients used yeast 5% (m/m) β-glucan ointment (study group) and Stratamed ointment (control), respectively, on a daily basis, for a maximum of four weeks. The size of the burn wounds was measured before and at the end of the treatment. Blood samples of 14 and 10 patients in the β-glucan and control groups, respectively, were obtained before and after the treatment, and the enzyme-linked immunosorbent assay (ELISA) was performed to measure the serum concentration of the IL-4, IL-17, and IFN-γ cytokines. The log-binomial model was used to assess the efficacy of the β-glucan ointment on burn wound healing. ANOVA/ANCOVA was employed to assess the effects of β-glucan on the serum concentration of cytokines. After adjusting for potential confounders/covariates, patients receiving β-glucan had better wound healing (RR = 4.34; 95% CI: 0.73 to 25.67; = 0.11). There was a significant difference in IL-4 secretion between the β-glucan and control groups after adjusting for potential confounders/covariates (MD = 77.27; 95% CI: 44.73 to 109.82; = 2.21; 95% CI: 1.16 to 3.24; = 0.0001). The results indicate that 5% (m/m) of β-glucan has efficacy in burn wound healing, and a significant difference was found in the level of IL-4 after receiving β-glucan. Further studies with a two-arm design and long-term use of ointment are needed to confirm the effect of β-glucan on wound healing and cytokine secretion.
烧伤患者的死亡率和伤残调整生命年（DALYs）随时间推移而下降。然而，人们高度重视寻找使用天然物质的新型有效治疗方法，以减轻烧伤负担。伤口愈合中最近使用的物质之一是β-葡聚糖，主要从真菌细胞壁中提取。本研究旨在评估5%（m/m）酵母β-葡聚糖软膏对烧伤创面愈合的影响，并评估治疗期间β-葡聚糖对细胞因子的影响。33例二度或三度烧伤患者纳入本研究。两组分别为23例和10例患者，每天分别使用5%（m/m）酵母β-葡聚糖软膏（研究组）和Stratamed软膏（对照组），最长使用四周。在治疗前和治疗结束时测量烧伤创面的大小。分别在β-葡聚糖组和对照组中，于治疗前和治疗后采集14例和10例患者的血样，采用酶联免疫吸附测定（ELISA）法检测IL-4、IL-17和IFN-γ细胞因子的血清浓度。采用对数二项模型评估β-葡聚糖软膏对烧伤创面愈合的疗效。采用方差分析/协方差分析评估β-葡聚糖对细胞因子血清浓度的影响。在调整潜在混杂因素/协变量后，接受β-葡聚糖治疗的患者伤口愈合情况更好（RR = 4.34；95%CI：0.73至25.67；P = 0.11）。在调整潜在混杂因素/协变量后，β-葡聚糖组和对照组之间的IL-4分泌存在显著差异（MD = 77.27；95%CI：44.73至109.82；P = 2.21；95%CI：1.16至3.24；P = 0.0001）。结果表明,5%（m/m）β-葡聚糖对烧伤创面愈合有效，接受β-葡聚糖治疗后IL-4水平存在显著差异。需要采用双臂设计并长期使用软膏进行进一步研究，以证实β-葡聚糖对伤口愈合和细胞因子分泌的影响。
