Batty I R, Nahorski S R, Irvine R F
Biochem J. 1985 Nov 15;232(1):211-5. doi: 10.1042/bj2320211.
Carbachol stimulation of muscarinic receptors in rat cortical slices prelabelled with myo-[2-3H]inositol caused the rapid formation of a novel inositol polyphosphate. Evidence derived from its chromatographic behaviour, and from the structure of the products formed in partial dephosphorylation experiments, suggests that it is probably D-myo-inositol 1,3,4,5-tetrakisphosphate. An enzyme in human red cell membranes specifically removes the 5-phosphate from it to form inositol 1,3,4-trisphosphate. It is suggested that inositol 1,3,4,5-tetrakisphosphate is likely to be a second messenger, and that it is the precursor of inositol 1,3,4-trisphosphate and possibly of inositol 1,4,5-trisphosphate.
用肌醇-[2-³H]标记的大鼠皮层切片中的毒蕈碱受体受到卡巴胆碱刺激后，迅速形成一种新型肌醇多磷酸。从其色谱行为以及部分去磷酸化实验中形成的产物结构获得的证据表明，它可能是D-肌醇1,3,4,5-四磷酸。人红细胞膜中的一种酶能特异性地从其上除去5-磷酸，形成肌醇1,3,4-三磷酸。有人提出，肌醇1,3,4,5-四磷酸可能是一种第二信使，并且它是肌醇1,3,4-三磷酸以及可能是肌醇1,4,5-三磷酸的前体。
