Giri Sapna P, Varma Sushilkumar B
J Basic Clin Physiol Pharmacol. 2015 Sep;26(5):479-84. doi: 10.1515/jbcpp-2014-0043.
The objective of this work was to study the analgesic and anti-inflammatory activity of Tectona grandis Linn. (TG) stem extract.
Healthy albino rats (150-200 g) and mice (25-50 g) were arranged and divided into five groups. Group 1 received distilled water and served as control, groups 2, 3, 4 were treated with TG stem extract (100, 200, 400 mg/kg) and group 5 received standard drug. For analgesic activity, the radiant heat method and writhing test were used, and for anti-inflammatory activity, the paw oedema model and granuloma pouch method were used. Statistical analysis was done by one-way analysis of variance followed by the t-test.
In the radiant heat method, there was a dose-dependent increase in reaction time in TG stem extract (100, 200, 400 mg/kg) and standard drug (buprenorphine). In the writhing test, TG stem extract (200, 400 mg/kg) and aspirin decreased the number of writhes which was significant (p<0.01, p<0.001) as compared to controls. The percentage protection was 8.88%, 15.55%, 22.22% and 35.55% in groups 2, 3, 4 and 5, respectively. In the rat paw oedema test, TG stem extract (200, 400 mg/kg) and aspirin showed 20%, 40% and 54% inhibition of oedema which was statistically significant (p<0.05, p<0.01, p<0.001), respectively, as compared to control at the end of 4 h. In the granuloma pouch method, TG stem extract (100, 200, 400 mg/kg) and aspirin showed 13.33%, 22.22%, 33.33% and 66.66% inhibition of granuloma, respectively, which was significant (p<0.05, p<0.01, p<0.001).
TG stem extract possesses significant anti-inflammatory and analgesic activity.
本研究旨在探讨柚木（TG）茎提取物的镇痛和抗炎活性。
选取健康的白化大鼠（150 - 200 g）和小鼠（25 - 50 g），分为五组。第1组给予蒸馏水作为对照，第2、3、4组分别用TG茎提取物（100、200、400 mg/kg）处理，第5组给予标准药物。采用辐射热法和扭体试验检测镇痛活性，采用足趾肿胀模型和肉芽肿袋法检测抗炎活性。采用单因素方差分析和t检验进行统计学分析。
在辐射热法中，TG茎提取物（100、200、400 mg/kg）和标准药物（丁丙诺啡）的反应时间呈剂量依赖性增加。在扭体试验中，TG茎提取物（200、400 mg/kg）和阿司匹林均能减少扭体次数，与对照组相比差异有统计学意义（p<0.01，p<0.001）。第2、3、4、5组的保护率分别为8.88%、15.55%、22.22%和35.55%。在大鼠足趾肿胀试验中，TG茎提取物（200、400 mg/kg）和阿司匹林在4小时末对肿胀的抑制率分别为20%、40%和54%，与对照组相比差异有统计学意义（p<0.05，p<0.01，p<0.001）。在肉芽肿袋法中，TG茎提取物（100、200、400 mg/kg）和阿司匹林对肉芽肿的抑制率分别为13.33%、22.22%、33.33%和66.66%，差异有统计学意义（p<0.05，p<0.01，p<0.001）。
TG茎提取物具有显著的抗炎和镇痛活性。
