Department of Nursing, Asia University, Taichung, Taiwan.
Intensive Care Unit, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan.
World J Surg. 2018 Jun;42(6):1714-1720. doi: 10.1007/s00268-017-4404-x.
The Omega-3 (ω-3) polyunsaturated fatty acids (PUFAs) generate bioactive lipid mediators that reduce inflammation. The present study evaluated the effect of SMOFlipid containing ω-3 PUFAs on wound healing.
Rats were divided into a SMOFlipid (SMOF) group and a 0.9% saline (placebo) group, with eight rats in each group. Wound excision was performed on the dorsal surface of each rat. In the SMOF group, 1 gm/kg SMOFlipid was dissolved in 3 mL saline as a treatment; in the placebo group, 3 mL saline was prepared as a treatment. The treatments were administered intravenously at an initial rate of 0.2 mL/kg body weight/h immediately after wounding, for 72 h. Blood samples were collected for white blood cell, tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 measurements at the baseline and at 1, 6, 12, 24, 48, and 72 h after intervention. Wound areas were measured over a 2-week period after excision, and a histological examination was performed.
Compared with the placebo group, SMOFlipid supplementation engendered significant decreases in the wound area on day 3 (78.28 ± 5.25 vs. 105.86 ± 8.89%), day 5 (72.20 ± 4.31 vs. 96.39 ± 4.72%), day 10 (20.78 ± 1.28 vs. 39.80 ± 10.38%), and day 14 (7.56 ± 0.61 vs. 15.10 ± 2.42%). The placebo group had a higher TNF-α level than the SMOF group at 72 h. The IL-10 level was higher in the SMOF group than in the placebo group at 48 h. Histological analysis revealed a higher rate of fibroblast distribution and collagen fiber organization in the SMOF group (P = 0.01).
SMOFlipid enriched in ω-3 PUFA accelerates wound healing.
ω-3 多不饱和脂肪酸（PUFA）可生成生物活性脂质介质，从而减轻炎症。本研究评估了含有ω-3 PUFA 的 SMOFlipid 对伤口愈合的影响。
将大鼠分为 SMOFlipid（SMOF）组和 0.9%生理盐水（安慰剂）组，每组 8 只大鼠。在每只大鼠的背部表面进行伤口切除。在 SMOF 组中，将 1 g/kg SMOFlipid 溶解在 3 mL 盐水中作为治疗药物；在安慰剂组中，制备 3 mL 生理盐水作为治疗药物。在创伤后立即以 0.2 mL/kg 体重/h 的初始速度静脉内给予治疗，持续 72 h。在干预后 1、6、12、24、48 和 72 h 时采集血液样本，用于测量白细胞、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6 和 IL-10。在切除后 2 周内测量伤口面积，并进行组织学检查。
与安慰剂组相比，SMOF 补充剂可显著减少第 3 天（78.28 ± 5.25 对 105.86 ± 8.89%）、第 5 天（72.20 ± 4.31 对 96.39 ± 4.72%）、第 10 天（20.78 ± 1.28 对 39.80 ± 10.38%）和第 14 天（7.56 ± 0.61 对 15.10 ± 2.42%）的伤口面积。安慰剂组在 72 h 时 TNF-α 水平高于 SMOF 组。SMOF 组在 48 h 时 IL-10 水平高于安慰剂组。组织学分析显示，SMOF 组的成纤维细胞分布和胶原纤维组织的比例更高（P=0.01）。
富含 ω-3 PUFA 的 SMOFlipid 可加速伤口愈合。
