Ding Xiang, Hou Yiling, Zhu Yuanxiu, Wang Panpan, Fu Lei, Zhu Hongqing, Zhang Nan, Qin Hang, Qu Wei, Wang Fang, Hou Wanru
Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, Nanchong, Sichuan 637009, P.R. China.
Oncol Rep. 2015 Jun;33(6):3162-70. doi: 10.3892/or.2015.3921. Epub 2015 Apr 21.
A novel heteropolysaccharide from the fruiting bodies of Gomphus clavatus Gray was isolated through Sephadex G-200 and DEAE-cellulose columns. The Gomphus clavatus Gray polysaccharide (GCG-1) was mainly composed of β-D-glucosepyranose (β-D-Glu) and α-D-galactopyranose (α-D-Gal) in a ratio of 3:2 and had a molecular weight of ~50,000 Da. The structure of GCG-1 was investigated by a combination of total hydrolysis, gas chromatography-mass spectrometry, methylation analysis, nuclear magnetic resonance spectroscopy and infrared spectra. The results indicated that GCG-1 had a backbone of (1 → 4)-β-D-glucosepyranose residues with branches at O-6 and the branches consisted of two with (1 → 3)-α-D-galactopyranose residue. Antioxidation test in vitro showed that it possessed strong free radical scavenging activity, which may be comparable to vitamin C and butylated hydroxytoluene. GCG-1 also induced the apoptosis of HepG-2 cells and affected the mRNA expression of various housekeeping genes in the HepG-2 cells. The results indicated that Gomphus clavatus Gray may be an ideal sources for antioxidant and anticancer agents.
通过Sephadex G - 200和DEAE - 纤维素柱从棒柄杯伞（Gomphus clavatus Gray）子实体中分离出一种新型杂多糖。棒柄杯伞多糖（GCG - 1）主要由β - D - 吡喃葡萄糖（β - D - Glu）和α - D - 吡喃半乳糖（α - D - Gal）以3:2的比例组成，分子量约为50,000 Da。通过全水解、气相色谱 - 质谱联用、甲基化分析、核磁共振光谱和红外光谱相结合的方法对GCG - 1的结构进行了研究。结果表明，GCG - 1具有由（1→4）-β - D - 吡喃葡萄糖残基组成的主链，在O - 6处有分支，分支由两个带有（1→3）-α - D - 吡喃半乳糖残基的结构组成。体外抗氧化试验表明，它具有较强的自由基清除活性，可能与维生素C和丁基羟基甲苯相当。GCG - 1还诱导了HepG - 2细胞的凋亡，并影响了HepG - 2细胞中各种管家基因的mRNA表达。结果表明，棒柄杯伞可能是抗氧化剂和抗癌剂的理想来源。
