Siddiqui Fariha Masood, Akram Muhammad, Noureen Nighat, Noreen Zobia, Bokhari Habib
Department of Biosciences, COMSATS Institute of Information Technology, Park Road, 44000, Islamabad, Pakistan.
Department of Biosciences, COMSATS Institute of Information Technology, Park Road, 44000, Islamabad, Pakistan.
Asian Pac J Trop Med. 2015 Mar;8(3):197-202. doi: 10.1016/S1995-7645(14)60314-X.
To determine antibiotic resistance patterns and virulence potential of Campylobacter jejuni (C. jejuni) isolates from clinical human diarrheal infections, cattle and healthy broilers.
Antibiotic sensitivity patterns of C. jejuni isolates were determined by Kirby Bauer Disc Diffusion assay. These isolates were then subjected to virulence profiling for the detection of mapA (membrane-associated protein), cadF (fibronectin binding protein), wlaN (beta-l,3-galactosyltransferase) and neuAB (sialic acid biosynthesis gene). Further C. jejuni isolates were grouped by random amplification of polymorphic DNA (RAPD) profiling.
A total of 436 samples from poultry (n=88), cattle (n=216) and humans (n=132) from different locations were collected. Results revealed percentage of C. jejuni isolates were 35.2% (31/88), 25.0% (54/216) and 11.3% (15/132) among poultry, cattle and clinical human samples respectively. Antibiotic susceptibility results showed that similar resistance patterns to cephalothin was ie. 87.0%, 87.1% and 89%among humans, poultry and cattle respectively, followed by sulfamethoxazole+trimethoprim 40.0%, 38.7% and 31.0% in humans, poultry and cattle and Ampicillin 40%, 32% and 20% in humans, poultry and cattle respectively. Beta-lactamase activity was detected in 40.00% humans, 20.37% cattle and 32.25% in poultry C. jejuni isolates. CadF and mapA were present in all poultry, cattle and human C. jejuni isolates, wlaN was not detected in any isolate and neuAB was found in 9/31 (36%) poultry isolates. RAPD profiling results suggested high diversity of C. jejuni isolates.
Detection of multidrug resistant C. jejuni strains from poultry and cattle is alarming as they can be potential hazard to humans. Moreover, predominant association of virulence factors, cadF and mapA (100% each) in C. jejuni isolates from all sources and neuAB (36%) with poultry isolates suggest the potential source of transmission of diverse types of C. jejuni to humans.
确定从临床人类腹泻感染、牛和健康肉鸡中分离出的空肠弯曲菌（C. jejuni）的抗生素耐药模式和毒力潜力。
采用 Kirby Bauer 纸片扩散法测定空肠弯曲菌分离株的抗生素敏感性模式。然后对这些分离株进行毒力分析，以检测mapA（膜相关蛋白）、cadF（纤连蛋白结合蛋白）、wlaN（β-1,3-半乳糖基转移酶）和neuAB（唾液酸生物合成基因）。进一步通过随机扩增多态性DNA（RAPD）分析对空肠弯曲菌分离株进行分组。
共收集了来自不同地点的家禽（n = 88）、牛（n = 216）和人类（n = 132）的436份样本。结果显示，家禽、牛和临床人类样本中空肠弯曲菌分离株的百分比分别为35.2%（31/88）、25.0%（54/216）和11.3%（15/132）。抗生素敏感性结果表明，对头孢噻吩的耐药模式相似，即人类、家禽和牛中的耐药率分别为87.0%、87.1%和89%，其次是磺胺甲恶唑+甲氧苄啶，人类、家禽和牛中的耐药率分别为40.0%、38.7%和31.0%，氨苄西林在人类、家禽和牛中的耐药率分别为40%、32%和20%。在人类空肠弯曲菌分离株中检测到β-内酰胺酶活性的占40.00%，牛中占20.37%，家禽中占32.25%。所有家禽、牛和人类空肠弯曲菌分离株中均存在CadF和mapA，未在任何分离株中检测到wlaN，在9/31（36%）的家禽分离株中发现了neuAB。RAPD分析结果表明空肠弯曲菌分离株具有高度多样性。
在家禽和牛中检测到多重耐药的空肠弯曲菌菌株令人担忧，因为它们可能对人类构成潜在危害。此外，所有来源的空肠弯曲菌分离株中毒力因子cadF和mapA（各占100%）以及家禽分离株中neuAB（36%）的主要关联表明，多种类型的空肠弯曲菌可能是传播给人类的潜在来源。
