Mi Yan, Zhang Chundong, Bu Youquan, Zhang Ying, He Longxia, Li Hongxia, Zhu Huifang, Li Yi, Lei Yunlong, Zhu Jiang
Department of Otolaryngology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Department of Biochemistry and Molecular Biology, Chongqing Medical University; Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, China.
BMB Rep. 2015 Jul;48(7):413-8. doi: 10.5483/bmbrep.2015.48.7.036.
DEPDC1 is a recently identified novel tumor-related gene that is upregulated in several types of cancer and contributes to tumorigenesis. In this study, we have investigated the expression pattern and functional implications of DEPDC1 during cell cycle progression. Expression studies using synchronized cells demonstrated that DEPDC1 is highly expressed in the mitotic phase of the cell cycle. Immunofluorescence assays showed that DEPDC1 is predominantly localized in the nucleus during interphase and is redistributed into the whole cell upon nuclear membrane breakdown in metaphase. Subsequently, siRNA-mediated knockdown of DEPDC1 caused a significant mitotic arrest. Moreover, knockdown of DEPDC1 resulted in remarkable mitotic defects such as abnormal multiple nuclei and multipolar spindle structures accompanied by the upregulation of the A20 gene as well as several cell cycle-related genes such as CCNB1 and CCNB2. Taken together, our current observations strongly suggest that this novel cancerous gene, DEPDC1, plays a pivotal role in the regulation of proper mitotic progression.
DEPDC1是最近发现的一种新型肿瘤相关基因，在多种癌症中上调并促进肿瘤发生。在本研究中，我们研究了DEPDC1在细胞周期进程中的表达模式及其功能意义。使用同步化细胞的表达研究表明，DEPDC1在细胞周期的有丝分裂期高度表达。免疫荧光分析显示，DEPDC1在间期主要定位于细胞核，在中期核膜破裂时重新分布到整个细胞中。随后，siRNA介导的DEPDC1敲低导致显著的有丝分裂停滞。此外，DEPDC1的敲低导致明显的有丝分裂缺陷，如异常多核和多极纺锤体结构，同时伴随着A20基因以及几个细胞周期相关基因（如CCNB1和CCNB2）的上调。综上所述，我们目前的观察结果强烈表明，这种新型癌基因DEPDC1在正常有丝分裂进程的调控中起关键作用。
