Department of Oncology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
Central Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
Cancer Biomark. 2021;30(3):299-307. doi: 10.3233/CBM-201760.
Previous studies revealed that DEP domain containing 1 (DEPDC1) is involved in the carcinogenesis and progression of several types of human cancer. However the role of DEPDC1 in gastric cancer has not been studied.
The objective of this study was to study the expression and pathophysiological function of DEPDC1 in gastric cancer.
DEPDC1 expression in gastric adenocarcinoma cells was examined with Western blot and qRT-PCR. Clinical pathological features of patients were determined by immunohistochemistry. The effect of DEPDC1 expression on cell proliferation was studied by in vitro cell proliferation assay; and cell cycle influence was assessed by flow cytometry. Survival curves were plotted using Kaplan-Meier.
DEPDC1 was overexpressed in gastric adenocarcinoma tissues compared with the paired adjacent normal gastric tissues, in accordance with mRNA level downloaded from GEPIA database. DEPDC1 expression level was significantly associated with cancer metastasis and differentiation. DEPDC1 upregulation caused cell cycle accelerating from G1 to S phase, and it was correlated with poorer overall survival.
Therefore, DEPDC1 upregulation in gastric adenocarcinoma is associated with tumor development and poor clinical outcomes of the patients, implying DEPDC1 might be a potential therapeutic target against gastric cancer.
先前的研究表明 DEP 结构域包含蛋白 1（DEPDC1）参与了几种类型人类癌症的发生和进展。然而，DEPDC1 在胃癌中的作用尚未得到研究。
本研究旨在研究 DEPDC1 在胃癌中的表达和病理生理功能。
采用 Western blot 和 qRT-PCR 检测胃腺癌细胞中 DEPDC1 的表达。通过免疫组织化学检测患者的临床病理特征。通过体外细胞增殖试验研究 DEPDC1 表达对细胞增殖的影响；通过流式细胞术评估细胞周期的影响。使用 Kaplan-Meier 绘制生存曲线。
与从 GEPIA 数据库下载的 mRNA 水平相比，DEPDC1 在胃腺癌组织中过度表达，与配对的相邻正常胃组织相比。DEPDC1 表达水平与癌症转移和分化显著相关。DEPDC1 的上调导致细胞周期从 G1 加速到 S 期，与整体生存较差相关。
因此，胃腺癌中 DEPDC1 的上调与肿瘤的发展和患者的不良临床结局相关，这表明 DEPDC1 可能是一种针对胃癌的潜在治疗靶点。
