长链非编码RNA GAS5影响细胞增殖并预示结直肠癌患者预后不良。
Long noncoding RNA GAS5 affects cell proliferation and predicts a poor prognosis in patients with colorectal cancer.
作者信息
Yin Dandan, He Xuezhi, Zhang Erbao, Kong Rong, De Wei, Zhang Zhihong
机构信息
Cancer Research and Therapy Center, The Second Affiliated Hospital of Southeast University, Nanjing, Jiangsu, China.
出版信息
Med Oncol. 2014 Nov;31(11):253. doi: 10.1007/s12032-014-0253-8. Epub 2014 Oct 18.
Colorectal cancer (CRC) is the third most common type of cancer worldwide. Recent studies have shown that lncRNAs play important roles in carcinogenesis. The aim of this study was to explore the role of lncRNA GAS5 in CRC. Real-time PCR was performed to investigate the expression of GAS5 in tumor tissues and corresponding non-tumor colorectal tissues from 66 patients with CRC. The lower expression of GAS5 was significantly correlated with large tumor size, low histological grade and advanced TNM stage. Multivariate analyses revealed that GAS5 expression served as an independent predictor for overall survival (P = 0.034). Further experiments revealed that overexpressed GAS5 significantly repressed the proliferation both in vitro and in vivo. In conclusion, our results suggest that GAS5, as a growth regulator, may serve as a candidate prognostic biomarker in human colorectal cancer.
结直肠癌（CRC）是全球第三大常见癌症类型。最近的研究表明，长链非编码RNA（lncRNAs）在癌症发生过程中发挥着重要作用。本研究的目的是探讨lncRNA GAS5在结直肠癌中的作用。对66例结直肠癌患者的肿瘤组织及相应的非肿瘤结直肠组织进行实时定量聚合酶链反应（Real-time PCR），以研究GAS5的表达情况。GAS5的低表达与肿瘤体积大、组织学分级低及TNM分期晚显著相关。多因素分析显示，GAS5表达是总生存期的独立预测指标（P = 0.034）。进一步实验表明，过表达GAS5在体外和体内均显著抑制增殖。总之，我们的结果表明，GAS5作为一种生长调节因子，可能是人类结直肠癌的候选预后生物标志物。
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