Choi Hoseong, Jo Yeonhwa, Lian Sen, Jo Kyoung-Min, Chu Hyosub, Yoon Ju-Yeon, Choi Seung-Kook, Kim Kook-Hyung, Cho Won Kyong
Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea.
Plant Mol Biol. 2015 Jun;88(3):233-48. doi: 10.1007/s11103-015-0317-y. Epub 2015 Apr 23.
The chrysanthemum is one of popular flowers in the world and a host for several viruses. So far, molecular interaction studies between the chrysanthemum and viruses are limited. In this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV) and Potato virus X (PVX). A chrysanthemum 135K microarray derived from expressed sequence tags was successfully applied for the expression profiles of the chrysanthemum at early stage of virus infection. Finally, we identified a total of 125, 70 and 124 differentially expressed genes (DEGs) for CMV, TSWV and PVX, respectively. Many DEGs were virus specific; however, 33 DEGs were commonly regulated by three viruses. Gene ontology (GO) enrichment analysis identified a total of 132 GO terms, and of them, six GO terms related stress response and MCM complex were commonly identified for three viruses. Several genes functioning in stress response such as chitin response and ethylene mediated signaling pathway were up-regulated indicating their involvement in establishment of host immune system. In particular, TSWV infection significantly down-regulated genes related to DNA metabolic process including DNA replication, chromatin organization, histone modification and cytokinesis, and they are mostly targeted to nucleosome and MCM complex. Taken together, our comparative transcriptome analysis revealed several genes related to hormone mediated viral stress response and DNA modification. The identified chrysanthemums genes could be good candidates for further functional study associated with resistant to various plant viruses.
菊花是世界上广受欢迎的花卉之一，也是几种病毒的宿主。到目前为止，菊花与病毒之间的分子相互作用研究还很有限。在本研究中，我们对菊花进行了转录组分析，以研究其对三种不同病毒的反应，这三种病毒分别是黄瓜花叶病毒（CMV）、番茄斑萎病毒（TSWV）和马铃薯X病毒（PVX）。一个源自表达序列标签的菊花135K微阵列成功应用于病毒感染早期菊花的表达谱分析。最后，我们分别鉴定出CMV、TSWV和PVX的差异表达基因（DEG）共125个、70个和124个。许多差异表达基因具有病毒特异性；然而，有33个差异表达基因受三种病毒共同调控。基因本体（GO）富集分析共鉴定出132个GO术语，其中，与应激反应和MCM复合体相关的6个GO术语在三种病毒中均被共同鉴定出来。一些在应激反应中起作用的基因，如几丁质反应和乙烯介导的信号通路相关基因被上调，表明它们参与了宿主免疫系统的建立。特别是，TSWV感染显著下调了与DNA代谢过程相关的基因，包括DNA复制、染色质组织、组蛋白修饰和胞质分裂，这些基因大多靶向核小体和MCM复合体。综上所述，我们的比较转录组分析揭示了几个与激素介导的病毒应激反应和DNA修饰相关的基因。所鉴定的菊花基因可能是进一步开展与抗多种植物病毒相关功能研究的良好候选基因。
