Lewis Nicola S, Verhagen Josanne H, Javakhishvili Zurab, Russell Colin A, Lexmond Pascal, Westgeest Kim B, Bestebroer Theo M, Halpin Rebecca A, Lin Xudong, Ransier Amy, Fedorova Nadia B, Stockwell Timothy B, Latorre-Margalef Neus, Olsen Björn, Smith Gavin, Bahl Justin, Wentworth David E, Waldenström Jonas, Fouchier Ron A M, de Graaf Miranda
Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK.
Department of Viroscience, Erasmus MC, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.
J Gen Virol. 2015 Aug;96(8):2050-2060. doi: 10.1099/vir.0.000155. Epub 2015 Apr 22.
Low pathogenic avian influenza A viruses (IAVs) have a natural host reservoir in wild waterbirds and the potential to spread to other host species. Here, we investigated the evolutionary, spatial and temporal dynamics of avian IAVs in Eurasian wild birds. We used whole-genome sequences collected as part of an intensive long-term Eurasian wild bird surveillance study, and combined this genetic data with temporal and spatial information to explore the virus evolutionary dynamics. Frequent reassortment and co-circulating lineages were observed for all eight genomic RNA segments over time. There was no apparent species-specific effect on the diversity of the avian IAVs. There was a spatial and temporal relationship between the Eurasian sequences and significant viral migration of avian IAVs from West Eurasia towards Central Eurasia. The observed viral migration patterns differed between segments. Furthermore, we discuss the challenges faced when analysing these surveillance and sequence data, and the caveats to be borne in mind when drawing conclusions from the apparent results of such analyses.
低致病性甲型禽流感病毒（IAVs）在野生水鸟中有天然宿主库，并有可能传播到其他宿主物种。在此，我们研究了欧亚野生鸟类中禽流感病毒的进化、空间和时间动态。我们使用了作为一项密集的长期欧亚野生鸟类监测研究的一部分收集的全基因组序列，并将这些遗传数据与时间和空间信息相结合，以探索病毒的进化动态。随着时间的推移，在所有八个基因组RNA片段中均观察到频繁的重配和共循环谱系。对于禽流感病毒的多样性，没有明显的物种特异性影响。欧亚序列之间存在时空关系，并且禽流感病毒有从西欧亚向中欧亚的显著病毒迁移。观察到的病毒迁移模式在不同片段之间有所不同。此外，我们讨论了分析这些监测和序列数据时面临的挑战，以及从这类分析的明显结果得出结论时应牢记的注意事项。
