Trumpaite-Vanagiene Rita, Bukelskiene Virginija, Aleksejuniene Jolanta, Puriene Alina, Baltriukiene Daiva, Rutkunas Vygandas
Institute of Odontology, Faculty of Medicine, Vilnius University.
Dent Mater J. 2015;34(3):294-301. doi: 10.4012/dmj.2014-185. Epub 2015 Apr 23.
The study aimed to 1) evaluate the cytotoxicity of luting cements: Hoffmann's Zinc Phosphate (Hoffmann's ZP), GC Fuji Plus Resin Modified Glass Ionomer (Fuji Plus RMGI) and 3M ESPE RelyX Unicem Resin Cement (RelyX Unicem RC) and 2) test if pre-washing reduces the cements' cytotoxicity. In vitro human gingival fibroblast (HGF) culture model was chosen. The cytotoxicity was evaluated by MTT test, the cell viability -by staining the cells with AO/EB dye mixture. The means±SD of Cell Survival Ratio (CSR%) were compared among different cement types under two testing conditions, with or without cement pre-washing. The CSR%s were compared by ANOVA and linear multiple regression (LMR). Hoffmann's ZPC was less cytotoxic, while Fuji Plus RMGIC and RelyX Unicem RC were more cytotoxic (ANOVA, p<0.001). The type of cement and cement pre-washing jointly explained 90% of cell survival (LMR, p<0.001, adjusted squared R=0.889). The commonly used luting cements such as Hoffmann's ZP, Fuji Plus RMGI and RelyX Unicem RC may have a cytotoxic potential.
1）评估粘结水门汀的细胞毒性：霍夫曼磷酸锌（Hoffmann's ZP）、GC富士Ⅱ玻璃离子水门汀（Fuji Plus RMGI）和3M ESPE RelyX Unicem树脂水门汀（RelyX Unicem RC）；2）测试预冲洗是否能降低水门汀的细胞毒性。选择体外人牙龈成纤维细胞（HGF）培养模型。通过MTT试验评估细胞毒性，通过用AO/EB染料混合物对细胞进行染色评估细胞活力。比较在两种测试条件下（有或没有水门汀预冲洗）不同水门汀类型之间的细胞存活率（CSR%）的平均值±标准差。通过方差分析（ANOVA）和线性多元回归（LMR）比较CSR%。霍夫曼磷酸锌水门汀的细胞毒性较小，而富士Ⅱ玻璃离子水门汀和RelyX Unicem树脂水门汀的细胞毒性较大（ANOVA，p<0.001）。水门汀类型和水门汀预冲洗共同解释了90%的细胞存活率（LMR，p<0.001，调整后的R平方=0.889）。常用的粘结水门汀，如霍夫曼磷酸锌、富士Ⅱ玻璃离子水门汀和RelyX Unicem树脂水门汀可能具有细胞毒性潜力。
