Faculty of Dentistry, Department of Prosthodontics, Ankara University, Ankara, Turkey.
Dental Materials Unit, Center for Dental and Oral Medicine, Clinic for Fixed and Removable Prosthodontics and Dental Materials Science, University of Zurich, Zurich, Switzerland.
Clin Oral Investig. 2020 Mar;24(3):1161-1170. doi: 10.1007/s00784-019-02980-3. Epub 2019 Jul 20.
This study evaluated the cytotoxicity of resin-based luting cements on fibroblast cells using different polymerization protocols.
Two conventional dual-polymerized (RelyX ARC, VariolinkN) and two self-adhesive resin cements (RelyX Unicem, Multilink Speed) specimens were polymerized using four different polymerization protocols: (a) photo-polymerization with direct light application, (b) photo-polymerization over ceramic and (c) resin nano-ceramic discs and (d) auto-polymerization. The specimens were then assigned to four groups to test cytotoxicity at 0, 1, 2 and 7 preincubation days (n = 5). MTT test was performed using NIH/3T3 fibroblast cells. Data were analysed using three- and one-way ANOVA. Multiple comparisons were made using Bonferroni post hoc test (p < 0.05).
The highest cytotoxic values were recorded at day 2 for conventional resin cements and at day 0 for self-adhesive resin cements. Self-adhesive resin cements showed the most cytotoxic effect at the second day, while conventional resin cements presented immediate cytotoxicity. Auto-polymerized resin specimens and especially Multilink Speed demonstrated the most cytotoxic effect regardless of the preincubation time. Cytotoxicity of cements tested reached the lowest level at day 7. Interposition of ceramic or nano-ceramic restorative material did not significantly affect the cytotoxicity of tested luting cements (p > 0.05).
Cytotoxicity of dual-polymerized resin cements was material-dependent and decreased gradually up to 7 days. Photo-polymerization plays an important role in reducing the cytotoxic effects.
When luting ceramic or resin nano-ceramic restorations of which thickness does not exceed 2 mm, the level of cytotoxicity with the tested materials is not significant. Luting of restorative materials that do not allow for light transmission such as metal-fused porcelain, clinicians should be cautious in the use of dual-polymerized conventional resin cements as only auto-polymerization of resin cements takes place under such materials.
本研究采用不同聚合方案评估了树脂水门汀对成纤维细胞的细胞毒性。
使用四种不同聚合方案聚合了两种传统的双重聚合（RelyX ARC、VariolinkN）和两种自粘接树脂水门汀（RelyX Unicem、Multilink Speed）试件：（a）直接光应用的光聚合，（b）光聚合在陶瓷上，（c）树脂纳米陶瓷片，（d）自动聚合。然后将试件分为四组，在 0、1、2 和 7 天预孵育后（n=5）进行细胞毒性测试。使用 NIH/3T3 成纤维细胞进行 MTT 试验。使用三因素和单因素方差分析分析数据。使用 Bonferroni 事后检验进行多重比较（p<0.05）。
传统树脂水门汀在第 2 天记录到最高的细胞毒性值，而自粘接树脂水门汀在第 0 天记录到最高的细胞毒性值。自粘接树脂水门汀在第 2 天表现出最具细胞毒性的作用，而传统树脂水门汀表现出即刻细胞毒性。自聚合树脂试件，尤其是 Multilink Speed，无论预孵育时间如何，都表现出最具细胞毒性的作用。测试用水泥的细胞毒性在第 7 天降至最低水平。陶瓷或纳米陶瓷修复材料的介入并没有显著影响测试用水门汀的细胞毒性（p>0.05）。
双重聚合树脂水门汀的细胞毒性取决于材料，并在 7 天内逐渐降低。光聚合在降低细胞毒性方面起着重要作用。
当粘固厚度不超过 2mm 的陶瓷或树脂纳米陶瓷修复体时，用测试材料的细胞毒性水平并不显著。对于不允许光透过的修复材料，如金属烤瓷熔附金属，临床医生在使用双重聚合传统树脂水门汀时应谨慎，因为只有在这种材料下才能进行树脂水门汀的自聚合。
