低危型人乳头瘤病毒 HPV6 和 HPV11 在布基纳法索喉乳头瘤病中的意义。

Implication of low risk human papillomaviruses, HPV6 and HPV11 in laryngeal papillomatosis in Burkina Faso.

机构信息

Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouaga I Professor Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso; Pietro Annigoni Biomolecular Research Center (CERBA), 01 BP 364, Ouagadougou 01, Burkina Faso.

University of Cape Coast, School of Medical Sciences, Department of Microbiology and Immunology, University Post Office, Ghana.

出版信息

Am J Otolaryngol. 2019 May-Jun;40(3):368-371. doi: 10.1016/j.amjoto.2019.02.003. Epub 2019 Feb 18.

DOI:10.1016/j.amjoto.2019.02.003

PMID:30799210

Abstract

PURPOSE

Laryngeal papillomatosis is the most common benign tumor of the larynx of children. It is characterized by the development of exophytic proliferative lesions in the mucosa of the airways. Human papillomavirus (HPV) has been recognized as a causal agent among which HPV types 6 and 11 are the most frequently implicated. This disease affects the vocal cords and other important functions of the child. The difficulty of treatment is related to the high recurrence of papilloma growth after surgical removal. The objective of this study was to describe the implication of HPV6 and HPV11 in cases of laryngeal papillomatosis histologically confirmed in Ouagadougou.

MATERIALS AND METHODS

This was a descriptive cross-sectional study based on histologically diagnosed archival tissue; obtained in the last ten years (2007 to 2017) in the anatomy and cyto-pathology laboratories in Burkina Faso. These fixed and paraffin-embedded tissues were deparaffinized with xylene before HPV DNA extraction; then HPV6 and HPV 11 were identified by real-time multiplex PCR.

RESULTS

The prevalence of low-risk HPV infection (HPV-LR) was 54.84% in histologically confirmed laryngeal papillomatosis in Ouagadougou. Among the HPV-LR positive samples, HPV6 and HPV11 genotype prevalence's were respectively 41.17% and 35.3% while the HPV6 / HPV11 co-infection was 23.53%.

CONCLUSIONS

The results show the implication of HPV6 and HPV11 in laryngeal papillomatosis in Burkina Faso with a high prevalence.

摘要

目的

喉乳头状瘤是儿童喉部最常见的良性肿瘤。其特征是气道黏膜外生性增生性病变的发展。人乳头瘤病毒（HPV）已被认为是一种致病因子，其中 HPV 6 型和 11 型最为常见。这种疾病会影响儿童的声带和其他重要功能。治疗困难与手术后乳头状瘤生长的高复发率有关。本研究的目的是描述 HPV6 和 HPV11 在布基纳法索瓦加杜古组织学确诊的喉乳头状瘤病例中的作用。