Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Int J Biol Macromol. 2015 Aug;79:95-102. doi: 10.1016/j.ijbiomac.2015.03.003. Epub 2015 Apr 20.
This work aimed at the development of biodegradable nanocapsules as carriers of two bioactive compounds, 5-aminosalycilic acid and glycomacropeptide. Nanocapsules were produced through layer-by-layer (LbL) deposition of chitosan (CH) and alginate (ALG) layers on polystyrene nanoparticles. The bioactive compounds were incorporated on the third layer of the nanocapsules being its encapsulation efficiency and release behaviour evaluated. The LbL deposition process, stability, morphology and size of the multilayer nanocapsules were monitored by means of zeta potential and transmission electron microscopy (TEM). The bioactive compounds release from the CH/ALG nanocapsules was successfully described by a mathematical model (linear superimposition model - LSM), which allowed concluding that bioactive compounds release is due to both Brownian motion and the polymer relaxation of the CH/ALG layers. Final results demonstrated that the synthesized LbL hollow nanocapsules presented spherical morphology and a good capacity to encapsulate different bioactive compounds, being the best results obtained for the system containing 5-aminosalycilic acid (with an encapsulation efficiency of approximately 70%). CH/ALG multilayer nanocapsules could be a promising carrier of bioactive compounds for applications in food and pharmaceutical industries.
本工作旨在开发可生物降解的纳米胶囊作为两种生物活性化合物（5-氨基水杨酸和糖巨肽）的载体。纳米胶囊是通过在聚苯乙烯纳米粒子上逐层（LbL）沉积壳聚糖（CH）和海藻酸钠（ALG）层来制备的。将生物活性化合物包封在纳米胶囊的第三层中，并评估其包封效率和释放行为。通过 zeta 电位和透射电子显微镜（TEM）监测 LbL 沉积过程、多层纳米胶囊的稳定性、形态和尺寸。通过数学模型（线性叠加模型-LSM）成功描述了生物活性化合物从 CH/ALG 纳米胶囊中的释放，该模型表明生物活性化合物的释放是由于 CH/ALG 层的布朗运动和聚合物松弛所致。最终结果表明，合成的 LbL 空心纳米胶囊具有球形形态和良好的包封不同生物活性化合物的能力，对于含有 5-氨基水杨酸的体系（包封效率约为 70%）获得了最佳结果。CH/ALG 多层纳米胶囊可能是一种有前途的生物活性化合物载体，可用于食品和制药行业。
