Roughan J V, Bertrand H G M J, Isles H M
Comparative Biology Centre, The Medical School, University of Newcastle, Newcastle upon Tyne, UK.
Eur J Pain. 2016 Feb;20(2):231-40. doi: 10.1002/ejp.712. Epub 2015 Apr 23.
Inflammation is thought to be a major contributor to post-surgical pain, so non-steroidal anti-inflammatory drugs (NSAIDs) are commonly used analgesics. However, compared to rats, considerably less is known as to how successfully these prevent pain in mice.
A fluorescent COX-2 selective probe was used for the first time to evaluate the post-surgical anti-inflammatory effects of meloxicam, and automated behaviour analyses (HomeCageScan; HCS), the Mouse Grimace Scale (MGS) and body weight changes to assess its pain-preventative properties. Groups of 8-9 BALB/c mice were subcutaneously injected with saline (0.3 mL) or meloxicam at (1, 5 or 20 mg/kg) 1 h before a 1.5-cm midline laparotomy. The probe or a control dye (2 mg/kg) was injected intravenously 3 h later. Imaging was used to quantify inflammation at 7, 24 and 48 h following surgery. HCS data and MGS scores were respectively obtained from video recordings and photographs before surgery and 24 h later.
Post-surgical inflammation was dose dependently reduced by meloxicam; with 5 or 20 mg/kg being most effective compared to saline. However, all mice lost weight, MGS scores increased and behavioural activity was reduced by surgery for at least 24 h with no perceivable beneficial effect of meloxicam on any of these potentially pain-associated changes.
Although meloxicam prevented inflammation, even large doses did not prevent post-laparotomy pain possibly arising due to a range of factors, including, but not limited to inflammation. MGS scoring can be applied by very naïve assessors and so should be effective for cage-side use.
炎症被认为是术后疼痛的主要促成因素，因此非甾体抗炎药（NSAIDs）是常用的镇痛药。然而，与大鼠相比，对于这些药物在小鼠中预防疼痛的效果了解甚少。
首次使用荧光COX-2选择性探针评估美洛昔康的术后抗炎作用，并采用自动行为分析（HomeCageScan；HCS）、小鼠 grimace 量表（MGS）和体重变化来评估其预防疼痛的特性。将8-9只BALB/c小鼠分为几组，在进行1.5厘米中线剖腹手术前1小时皮下注射生理盐水（0.3毫升）或美洛昔康（1、5或20毫克/千克）。3小时后静脉注射探针或对照染料（2毫克/千克）。在手术后7、24和48小时利用成像技术量化炎症。HCS数据和MGS评分分别从手术前和术后24小时的视频记录和照片中获取。
美洛昔康能剂量依赖性地减轻术后炎症；与生理盐水相比，5或20毫克/千克的剂量效果最佳。然而，所有小鼠体重均下降，MGS评分增加，并且手术使行为活动减少至少24小时，美洛昔康对这些任何潜在的疼痛相关变化均无明显有益作用。
尽管美洛昔康可预防炎症，但即使大剂量也无法预防剖腹手术后可能因一系列因素（包括但不限于炎症）引起的疼痛。MGS评分可由经验极少的评估者应用，因此应适用于笼旁使用。
