Ye Naihao, Zhang Xiaowen, Miao Miao, Fan Xiao, Zheng Yi, Xu Dong, Wang Jinfeng, Zhou Lin, Wang Dongsheng, Gao Yuan, Wang Yitao, Shi Wenyu, Ji Peifeng, Li Demao, Guan Zheng, Shao Changwei, Zhuang Zhimeng, Gao Zhengquan, Qi Ji, Zhao Fangqing
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
1] Computational Genomics Lab, Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China [2] College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Nat Commun. 2015 Apr 24;6:6986. doi: 10.1038/ncomms7986.
Seaweeds are essential for marine ecosystems and have immense economic value. Here we present a comprehensive analysis of the draft genome of Saccharina japonica, one of the most economically important seaweeds. The 537-Mb assembled genomic sequence covered 98.5% of the estimated genome, and 18,733 protein-coding genes are predicted and annotated. Gene families related to cell wall synthesis, halogen concentration, development and defence systems were expanded. Functional diversification of the mannuronan C-5-epimerase and haloperoxidase gene families provides insight into the evolutionary adaptation of polysaccharide biosynthesis and iodine antioxidation. Additional sequencing of seven cultivars and nine wild individuals reveal that the genetic diversity within wild populations is greater than among cultivars. All of the cultivars are descendants of a wild S. japonica accession showing limited admixture with S. longissima. This study represents an important advance toward improving yields and economic traits in Saccharina and provides an invaluable resource for plant genome studies.
海藻对海洋生态系统至关重要，且具有巨大的经济价值。在此，我们对经济上最重要的海藻之一——海带的基因组草图进行了全面分析。组装后的537兆碱基基因组序列覆盖了估计基因组的98.5%，并预测和注释了18733个蛋白质编码基因。与细胞壁合成、卤素浓度、发育和防御系统相关的基因家族有所扩展。甘露糖醛酸C-5-差向异构酶和卤过氧化物酶基因家族的功能多样化为多糖生物合成和碘抗氧化的进化适应性提供了见解。对7个栽培品种和9个野生个体的额外测序表明，野生种群内的遗传多样性大于栽培品种之间的遗传多样性。所有栽培品种均为一个野生海带种质的后代，与长海带的混合程度有限。这项研究代表了在提高海带产量和经济性状方面的一项重要进展，并为植物基因组研究提供了宝贵资源。
