Buttery J E, Chamberlain B R, Beng C G
Department of Clinical Chemistry, Queen Elizabeth Hospital, Woodville, South Australia.
Clin Chem. 1989 Dec;35(12):2311-2.
In this screening method for urinary porphobilinogen (PBG), urine is added to Dowex 2 resin under alkaline conditions in a test tube and mixed. The supernate is removed and the adsorbed PBG is eluted with acid and reacted with Ehrlich's reagent. We compared results with those by the Watson-Schwartz screening method, using urine samples from normal people with and without added PBG. At a PBG concentration of about five times the upper limit of normal, the resin method gave a sensitivity of 100%; the Watson-Schwartz method gave a sensitivity of 51%. At lower PBG concentrations of just over and twice the upper limit of normal, the sensitivity by the resin method was respectively 97% and 100%. With normal urine samples, the resin method gave negative results for all samples (100% specificity) and the Watson-Schwartz had 95% specificity. Our data indicate that the resin method is sensitive, specific, and reliable and is superior to the Watson-Schwartz method.
在这种尿卟胆原（PBG）筛查方法中，在试管的碱性条件下将尿液加入到Dowex 2树脂中并混合。去除上清液，用酸洗脱吸附的PBG，并与埃利希试剂反应。我们使用添加和未添加PBG的正常人尿液样本，将结果与沃森-施瓦茨筛查方法的结果进行比较。在PBG浓度约为正常上限的五倍时，树脂法的灵敏度为100%；沃森-施瓦茨法的灵敏度为51%。在略高于正常上限和两倍于正常上限的较低PBG浓度下，树脂法的灵敏度分别为97%和100%。对于正常尿液样本，树脂法对所有样本均给出阴性结果（特异性为100%），而沃森-施瓦茨法的特异性为95%。我们的数据表明，树脂法灵敏、特异且可靠，优于沃森-施瓦茨法。
