铁皮石斛多糖通过代谢组学分析预防小鼠乙醇性肝损伤。
Dendrobium huoshanense polysaccharide prevents ethanol-induced liver injury in mice by metabolomic analysis.
机构信息
School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China; Department of Pharmacy, The First Affiliated Hospital, Anhui University of Traditional Chinese Medicine, Hefei, China.
School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China.
出版信息
Int J Biol Macromol. 2015;78:354-62. doi: 10.1016/j.ijbiomac.2015.04.024. Epub 2015 Apr 22.
The prevalence of alcohol consumption has increased in modern dietary life and alcoholic liver injury can follow. Dendrobium huoshanense polysaccharide (DHP) is a homogeneous polysaccharide isolated from Dendrobium huoshanense, which possesses hepatoprotection function. In this study, we investigated the metabolic profiles of serum and liver tissues extracts from control, ethanol-treated and DHP\ethanol-treated mice using a UHPLC/LTQ Orbitrap XL MS-based metabolomics approach. Our results indicated that DHP alleviated early steatosis and inflammation in liver histology and the metabolomic analysis of serum and hepatic tissue revealed that first, ethanol treatment mainly altered phosphatidylcholines (PCs) including PC (13:0) and phosphocholine, arachidonic acid metabolites including 20-ethyl PGF2α and amino acids including L-Proline; Second, DHP supplementation ameliorated the altered metabolic levels particularly involved in phosphocholine and L-Proline. These data suggested that DHP might restore the perturbed metabolism pathways by ethanol exposure to prevent the progression of alcoholic liver injury.
现代饮食生活中，酒精消费的流行程度有所增加，随之而来的是酒精性肝损伤。霍山石斛多糖（DHP）是从霍山石斛中分离得到的均一多糖，具有保肝作用。本研究采用 UHPLC/LTQ Orbitrap XL MS 代谢组学方法，研究了对照、乙醇处理和 DHP+乙醇处理小鼠血清和肝组织提取物的代谢谱。结果表明，DHP 减轻了肝组织学中的早期脂肪变性和炎症，血清和肝组织的代谢组学分析表明，首先，乙醇处理主要改变了磷脂酰胆碱（PCs），包括 PC（13：0）和磷酸胆碱、花生四烯酸代谢物，包括 20-乙基 PGF2α 和氨基酸，包括 L-脯氨酸；其次，DHP 补充改善了代谢水平的变化，特别是磷酸胆碱和 L-脯氨酸。这些数据表明，DHP 可能通过恢复乙醇暴露引起的紊乱代谢途径来预防酒精性肝损伤的进展。
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