State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.
State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.
Int J Biol Macromol. 2020 Dec 1;164:1939-1948. doi: 10.1016/j.ijbiomac.2020.08.007. Epub 2020 Aug 5.
Type 2 diabetes (T2D) is a complicated endocrine metabolic disease, accompanied with oxidative stress injury and low-grade inflammation. The effects of polysaccharide extracted from Dendrobium officinale stem (DOP) on oxidative stress, inflammation and dysregulated metabolism in the liver of type 2 diabetic rats and its potential mechanism were evaluated in the study. Here, ultra performance liquid chromatography - quadrupole - time - of - flight (UPLC-Q-TOF) mass spectrometry-based lipidomics and metabolomics analysis were carried out to study the amelioration of DOP on the liver metabolism disorders of type 2 diabetic rats. Lipidomics analysis indicated that the disturbed degree of fatty acid, glycerolipid (diacylglycerol and triacylglycerol), and glycerophospholipid (phosphatidylcholine and phosphatidylethanolamine) metabolism were mitigated by the DOP treatment. Metabolomics analysis revealed that the DOP treatment balanced the metabolism of ceramide and bile acids, including deoxycholic acid, taurocholic acid, and cholic acid. In addition, the symptoms of oxidative stress, inflammation, and hepatic lipid accumulation of liver were ameliorated by DOP administration. The above results suggest that the study of functional ingredient application of DOP might be meaningful for the management of T2D.
2 型糖尿病（T2D）是一种复杂的内分泌代谢疾病，伴随着氧化应激损伤和低度炎症。本研究评估了铁皮石斛茎多糖（DOP）对 2 型糖尿病大鼠肝脏氧化应激、炎症和代谢失调的影响及其潜在机制。在这里，我们进行了基于超高效液相色谱 - 四极杆 - 飞行时间（UPLC-Q-TOF）质谱的脂质组学和代谢组学分析，以研究 DOP 对 2 型糖尿病大鼠肝脏代谢紊乱的改善作用。脂质组学分析表明，DOP 处理减轻了脂肪酸、甘油酯（二酰甘油和三酰甘油）和甘油磷脂（磷脂酰胆碱和磷脂酰乙醇胺）代谢的紊乱程度。代谢组学分析显示，DOP 处理平衡了神经酰胺和胆汁酸的代谢，包括脱氧胆酸、牛磺胆酸和胆酸。此外，DOP 给药改善了肝脏的氧化应激、炎症和脂质积累症状。上述结果表明，DOP 功能成分应用的研究对于 T2D 的管理可能具有重要意义。
