Rao Dan, Hu Yadong, Zhao Ruoxi, Li Hongjie, Chun Ze, Zheng Shigang
CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
University of Chinese Academy of Sciences, Beijing 100041, China.
Int J Anal Chem. 2022 Aug 19;2022:9510598. doi: 10.1155/2022/9510598. eCollection 2022.
is a beautiful orchid and a widely used medicinal plant. In vitro antioxidant assays suggested that showed significantly higher 2, 2'-azinobis-3-ethylbenzthiazoline-6-sulfonate (ABTS) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging rates and much more ferric-reducing power than those of root, stem, leaf and fruit. To better understand the antioxidant basis of flower, high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was used for metabolic identification and quantification. Finally, there were 72 metabolites among the total of 712 identified components showed significant association (coefficient >0.8, < 0.05) with ABTS scavenging rates, DPPH scavenging rates, and ferric-reducing power. The three enriched classes of flower metabolites, including amino acids and their derivatives, organic acids and their derivatives, and flavonoids, formed the main antioxidant basis. The significantly accumulated rutin, astragalin, isomucronulatol-7-O-glucoside, quercetin 4'-O-glucoside, methylquercetin O-hexoside, caffeic acid, caffeic acid O-glucoside, and -coumaric acid (Log(fold change) >2, < 0.01, distribution in flower >0.1%) made a key contribution to the higher antioxidant activities in flower. The relative quantification results of HPLC-MS/MS were verified by the common quantification methods. The antioxidant basis revealed of flower will be helpful in the production of healthy or beauty products.
是一种美丽的兰花，也是一种广泛使用的药用植物。体外抗氧化试验表明，其表现出比根、茎、叶和果实显著更高的2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)（ABTS）和1,1-二苯基-2-苦基肼（DPPH）清除率以及更强的铁还原能力。为了更好地理解该花的抗氧化基础，采用高效液相色谱串联质谱（HPLC-MS/MS）进行代谢物鉴定和定量分析。最终，在总共712种鉴定出的成分中，有72种代谢物与ABTS清除率、DPPH清除率和铁还原能力呈现显著相关性（系数>0.8，P<0.05）。花中的三类富集代谢物，包括氨基酸及其衍生物、有机酸及其衍生物和黄酮类化合物，构成了主要的抗氧化基础。显著积累的芦丁、黄芪苷、异鼠李素-7-O-葡萄糖苷、槲皮素4'-O-葡萄糖苷、甲基槲皮素O-己糖苷、咖啡酸、咖啡酸O-葡萄糖苷和对香豆酸（Log(倍数变化)>2，P<0.01，在花中的分布>0.1%）对花中较高的抗氧化活性起了关键作用。HPLC-MS/MS的相对定量结果通过常用定量方法进行了验证。揭示的该花的抗氧化基础将有助于健康或美容产品的生产。
